FastQCFastQC Report
Fri 30 Jun 2023
SRR1945004.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1945004.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23677484
Total Bases740.4 Mbp
Sequences flagged as poor quality0
Sequence length25-49
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGA427538018.056732717040376No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGA3963341.6738856206174608No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGA3404591.437901932485732No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAGA3001881.2678205167407146No Hit
ATAGCCCAACGCAAGGCGGGGTGCAATTATTAGA1552930.6558678278486008No Hit
ATATCCCAACGCAAGGCGGGGTGCAATTATTAGA1467820.6199222856617704No Hit
TCAATGAGTTGAATACAAATGATTCTTCGA1466530.6193774642609832No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAA1328490.5610773509550254No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGT871450.36805008505126646No Hit
TGTGGGTTCGTGGCGGCTAGCGTTTAGTT819850.3462572290197725No Hit
TCAATGAGTTGAATACAAATGATTCTTCG753680.3183108475545797No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAG600230.2535024413911546No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGT582390.24596785705778534No Hit
GTGGGTTCGTGGCGGCTAGCGTTTAGTT446380.18852509835926823No Hit
TTCAATGAGTTGAATACAAATGATTCTTCGA422310.17835932229961596No Hit
TTCAATGAGTTGAATACAAATGATTCTTCG419830.17731191371515656No Hit
ATGTACACGGAGTCGACCCGCAACGCGA402770.17010675627528668No Hit
TTTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTG392050.16557924820052672No Hit
ATTCCCCAACGCAAGGCGGGGTGCAATTATTAGA329710.1392504372508498No Hit
ATGCCCCAACGCAAGGCGGGGTGCAATTATTAGA326900.13806365574990992No Hit
TCTACGCCGACGGCGTTGGCTGCTCGTTCTAGC297320.1255707743272047No Hit
ACTTTGGTCGTGACTTCTAATTTGCTGGT276570.11680717427578031No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAG274740.11603428810257035No Hit
ATACCCCAACGCAAGGCGGGGGGCAATTATTAGA256180.108195617405971No Hit
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGA249360.10531524379870766No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)