FastQCFastQC Report
Fri 30 Jun 2023
SRR1945005.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1945005.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27850343
Total Bases859.6 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTGGTCGTGACTTCTAATTTGCTGGT403008514.470504007796242No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGT14448125.187770936968353No Hit
TGGCCTTTTTTCGAAGCATTAAGCGGAGCCA11831544.248256475692239No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAA10008103.5935284531325165No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATT9429463.3857608145077425No Hit
TCGGCCTTTTTTCGAAGCATTAAGCGGAGCCA9022663.2396943908374847No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTT6585912.3647500499365486No Hit
TCAACTGTGGTCGTGACTTCTAATTTGCTGGT6239172.2402488902919435No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGT5667232.0348869671012673No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAA5347201.9199763536125931No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGT4888071.755120215216021No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGG4354881.563671944722548No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCATT4323101.5522609542008152No Hit
TGGCCTTTTTTCGAAGCATTAAGCGGAGCCAT4029901.4469839743086825No Hit
CCCTTTTTTCGAAGCATTAAGCGGAGCCATT3900321.4004567196892332No Hit
TCTTTTTTCGAAGCATTAAGCGGAGCCATT3331301.1961432575534168No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGA3275271.1760250134082728No Hit
TCGCCTTTTTTCGAAGCATTAAGCGGAGCCA3157431.13371314672857No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTT2954471.06083792217568No Hit
TCAACTGTGGTCGTGACTTCTAATTTGCTGG2846591.0221023130666649No Hit
TACTGTGGTCGTGACTTCTAATTTGCTGGT2384800.8562910697365558No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCATT2381640.8551564338004741No Hit
TTAACTGTGGTCGTGACTTCTAATTTGCTGGT2222900.7981589311126257No Hit
TTGGCCTTTTTTCGAAGCATTAAGCGGAGCCA2214310.7950745884889101No Hit
TCGGCCTTTTTTCGAAGCATTAAGCGGAGCC1984200.7124508304978506No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGT1928130.6923182238725031No Hit
TCGCCTTTTTTCGAAGCATTAAGCGGAGCCAT1837810.659887743572853No Hit
TGGCCTTTTTTCGAAGCATTAAGCGGAGCC1829860.6570332006323943No Hit
TTTGTGCTTGCTTGCGGACGCTTTCTGGTG1620260.5817738043657128No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGG1609670.577971337731819No Hit
CCCTTTTTTCGAAGCATTAAGCGGAGCCAT1595440.5728618853994006No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGG1429950.5134407141772006No Hit
CAACTGTGGTCGTGACTTCTAATTTGCTGGT1425560.5118644319748593No Hit
TTGCGGACGCTTTCTGGTGTGTGCTTGGACC1366950.4908198078565854No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAA1243410.4464612877478744No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGTT1231910.4423320746893495No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGG1167940.4193628782237978No Hit
TTGTGCTTGCTTGCGGACGCTTTCTGGTG1150020.4129284870926006No Hit
CGGCCTTTTTTCGAAGCATTAAGCGGAGCCA1120840.40245105778409984No Hit
TGCGGACGCTTTCTGGTGTGTGCTTGGACC996650.35785914737208085No Hit
TCGGCCTTTTTTCGAAGCATTAAGCGGAGC965900.3468179907155901No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTTT813090.29194972571792027No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAC781260.2805207820959333No Hit
TTGGCCTTTTTTCGAAGCATTAAGCGGAGCCAT758160.2722264497783744No Hit
TTAACTGTGGTCGTGACTTCTAATTTGCTGG703790.2527042485616784No Hit
GCCTTTTTTCGAAGCATTAAGCGGAGCCA688220.24711365314244066No Hit
ATTGTGCTTGCTTGCGGACGCTTTCTGGT685450.24611905138834378No Hit
CCGGCCTTTTTTCGAAGCATTAAGCGGAGCCA660340.2371030044405557No Hit
GCCTTTTTTCGAAGCATTAAGCGGAGCCAT615180.22088776429073065No Hit
TGTCCTTTTTTCGAAGCATTAAGCGGAGCCA585540.21024516645988886No Hit
ACGGCCTTTTTTCGAAGCATTAAGCGGAGCCA551220.19792215844522992No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTT549050.1971429938941865No Hit
TCTGCCTTTTTTCGAAGCATTAAGCGGAGCCA548270.196862925530217No Hit
CCAACTGTGGTCGTGACTTCTAATTTGCTGGT525270.188604499413167No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAA523390.18792946284359946No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTT515490.1850928730033953No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAA510490.18329756297794966No Hit
GCGGCCTTTTTTCGAAGCATTAAGCGGAGCCA500740.17979670842833065No Hit
TTGCCTTTTTTCGAAGCATTAAGCGGAGCCA497570.17865848187219813No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCAT489200.1756531328896021No Hit
TGATTGTGCTTGCTTGCGGACGCTTTCTGGT452730.16255814156400156No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAACA446370.1602745072116347No Hit
AGGCCTTTTTTCGAAGCATTAAGCGGAGCCA442470.1588741653917871No Hit
ACGGTGGTCGTGACTTCTAATTTGCTGGT440270.158084228980591No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCAT427670.15356004771646797No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAGA426260.15305377028929232No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGTT412730.14819566136043638No Hit
CAACTGTGGTCGTGACTTCTAATTTGCTGG405200.14549192446211523No Hit
TACTGTGGTCGTGACTTCTAATTTGCTGGTT395300.14193721061173287No Hit
TAAACTGTGGTCGTGACTTCTAATTTGCTGGT384320.1379947097958542No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAC377780.1356464442825713No Hit
ATTGTGCTTGCTTGCGGACGCTTTCTGGTG361800.12990863344124703No Hit
TCGCCTTTTTTCGAAGCATTAAGCGGAGCC351340.12615284486801473No Hit
TTGGCCTTTTTTCGAAGCATTAAGCGGAGCC344780.12379739811463003No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGA344660.12375431067401935No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGA338350.12148862942190694No Hit
TCGGCCTGGGAATCCTGGATGTTGTAAGCT337240.12109007059625801No Hit
GCAACTGTGGTCGTGACTTCTAATTTGCTGGT326210.1171296166801249No Hit
TTGTGGTCGTGACTTCTAATTTGCTGGTT318880.1144976921828216No Hit
CCGCCTTTTTTCGAAGCATTAAGCGGAGCCA315980.11345641236806311No Hit
TGGCCTTTTTTCGAAGCATTAAGCGGAGC302100.108472631737426No Hit
CCGTGGCGACGCTGGTTCGTTTCCAGCCGCA290960.10447268100073309No Hit
TTGCGGACGCTTTCTGGTGTGTGCTTGGACCT287210.10312619848164886No Hit
GGGCCTTTTTTCGAAGCATTAAGCGGAGCCA286070.10271686779584725No Hit
TGCGGACGCTTTCTGGTGTGTGCTTGGACCT279770.10045477716378573No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)