FastQCFastQC Report
Sat 23 Sept 2023
SRR19641913.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19641913.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35602743
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG1508700.4237594839251571No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG1177900.3308452947010291No Hit
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1126550.3164222487014554No Hit
CGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA1064310.29894044961648036No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA910040.25560951862613507No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA788850.22157000655820255No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG781300.21944938343655152No Hit
CGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGAG772350.21693553218638237No Hit
CGGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA761440.21387116155628794No Hit
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA741900.20838282039111425No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG713040.2002767033989488No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA666350.18716254531287096No Hit
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA659900.18535088714934128No Hit
CGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG657360.18463745897331563No Hit
CCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG652360.18323307280003678No Hit
CCGCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAGATCG651560.18300837101231218No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG625620.17572241554534154No Hit
CCGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA619350.17396131528404987No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAA608710.17097278150731252No Hit
GGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGAG604370.16975377430890648No Hit
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550040.1544937141500586No Hit
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA538520.15125800840682416No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAA536260.15062322585650212No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG528640.14848294132842516No Hit
GCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA490870.13787420817547683No Hit
GCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG490650.13781241518385257No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAA481450.13522834462501948No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG476950.13396439706906854No Hit
GCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG474760.1333492759251724No Hit
GCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG472840.1328099916346333No Hit
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA472830.13280718286228677Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG465060.13062476674901144No Hit
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA451980.126950892519714No Hit
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA436150.1225046058951132Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG429720.12069856527627659No Hit
GGGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA424100.11912003521751119No Hit
GGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAGA417680.11731680337102116No Hit
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410400.11527201710272716No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAA405640.1139350414657657No Hit
CGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAGA399670.11225820437487077No Hit
CCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAGA384750.10806751603380671No Hit
CGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAGA384560.10801414935922213No Hit
GCGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA373410.10488236819281031No Hit
CGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAGA366550.10295555036307173No Hit
GCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAA363600.10212696252083722No Hit
GCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAA361010.10139949048307878No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG360070.10113546588250237No Hit
CGGCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAGATCG357950.10054000614503214No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)