FastQCFastQC Report
Sat 1 Jul 2023
SRR1964306.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1964306.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39082700
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAG10788652.7604669073528694No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCA6405551.6389732541508133No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAGC2134300.5460984015945674No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGC1372990.35130377379249644No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGC1312900.33592868455864106No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGCA1156940.29602356029649946No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAG926820.2371432884626702No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCCATGTCGTC850480.2176103493361513No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGCC817770.20924091733682676No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGCCTTGGCCG660310.16895199154613166No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN538170.13770031241444425No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCCGCCTTGGCCGTACAGCA432550.11067556745055997No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCGCCTT412880.10564265007279436No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)