FastQCFastQC Report
Thu 6 Jul 2023
SRR1964307.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1964307.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52274080
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAG16615693.1785714832283993No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCA9306591.7803450582009286No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAGC3905270.7470757974124078No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGC2725100.5213099876650149No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGC2269270.43410998337990836No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGCA2063610.39476734932494273No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAG1992110.3810894424158206No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGCC1772750.33912600661742875No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCTTGGCCGTACAGCAGCCTC1144850.219009115033684No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCCGCCTTGGCCGTACAGCA857230.16398758237352049No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCGCCTT778960.14901457854447175No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGCCTTGGCCG744940.14250657304729228No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCCTTGGCCGTA684580.13095974142443062No Hit
GTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACA583040.11153520061950398No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCCTTGG537590.10284064301083827No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)