FastQCFastQC Report
Fri 30 Jun 2023
SRR1964308.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1964308.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29216820
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAG13485614.615700818911846No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCA4143151.4180701390500403No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAGC1850570.6333919981709166No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGCA1595740.5461716915119441No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGC1394570.47731751778598763No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAG1084840.37130666513330335No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGC937080.320733057191029No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGCC868160.2971439054626753No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCGCCTT848510.2904183275250352No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCTTGGCCGTACAGCAGCCTC754590.25827246086329725No Hit
CCTCCCGGGGCTCATAAGTGAGTCTATGATAGATAAGGTTAATGCACTAC722180.24717953562365785No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGCCTTGGCCG700430.23973519363161355No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCCGCCTTGGCCGT586600.20077475919692833No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCCTTGGCCGTA456850.15636540869266402No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCCGCCTTGGCCGTACAGCA416150.14243507678111444No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCGCCT393720.1347579921428821No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCCTTGG378160.1294322927683437No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCCGCCTTGGCCGTACAGCAG361060.12357949975390888No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCGCCTTGGCCGTACAGCAGCC323270.11064516946060522No Hit
GTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCCGCCTTGGCCGTA318980.10917683717803649No Hit
GTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACA316340.1082732480810711No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACGCCTTGGCCGTACAGC305120.10443299441896825No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)