FastQCFastQC Report
Sat 1 Jul 2023
SRR1964309.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1964309.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46953344
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAG23853935.080347418918661No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCA5260151.1202929444173348No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGCA2443180.5203420655193376No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGC1965440.4185942539044717No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCTTGGCCGTACAGCAGGACC1502940.32009221749999317No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAGC1121060.23876041714941537No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCCATGTCGTC1074830.2289144730564877No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACGCCTTGGCCGTACAGC1032580.21991617892007861No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCGCCTT958370.20411112784640006No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGG884400.18835719134296378No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGCCTTGGCCG867820.18482602644872323No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACTCGTC836520.17815983457962015No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCCTTGGCCGTA765840.16310659364325575No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGGA666460.14194090201541343No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACTCGT584910.12457259700182377No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN567770.1209221647770178No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAG561220.11952716296415437No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCGCCTTGGCCGTACAGCAGGA524010.11160227480283406No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)