FastQCFastQC Report
Fri 30 Jun 2023
SRR1964310.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1964310.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67731496
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAG31835134.700195902951855No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCA8645371.2764179902360344No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGCA4032480.5953626064896013No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGC3661700.5406199798096885No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCTTGGCCGTACAGCAGGACC3350420.49466204024195776No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAGC2161910.31918828428062473No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCGCCTT1565530.23113766747452325No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGGA1523790.22497509873397747No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGG1452500.21444971479738173No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACGCCTTGGCCGTACAGC1333650.19690248684304862No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCCTTGGCCGTA1272990.18794653524262922No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAG1226480.18107971511510687No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACTCGTC871020.12859896081433075No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGCCTTGGCCG826900.12208500458929772No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCCTTGG821090.1212272057301082No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCTTGGCCGTACAGCAGGAC782170.11548098686613979No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCGCCTTGGCCGTACAGCAGGA743180.10972443307615708No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCCGCCTTGGCCGTACAGCA684600.10107557642016353No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCCGCCTTGGCCGTACAGCAG680850.10052191966939575No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)