FastQCFastQC Report
Thu 6 Jul 2023
SRR1964312.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1964312.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46189851
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAG36485077.898936500141557No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCA16744203.625082055363201No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGC3182270.6889543765793918No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGA3154840.6830158425927808No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGCA2066170.44732120915479895No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGAC1869300.40469929205876853No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAGC1512660.3274875253440415No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCCATGTCGTC885310.19166764577785714No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTATTCCAATTACGTGATG855600.18523549686271992No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAG775820.16796330431981693No Hit
CTCCCGGGATCGTATTCACTCAGATACTATCTATTCCAATTACGTGATGA774950.16777495125498454No Hit
CCTCCCGGGCGATGCGGACAGACTCGCAGCGAGCGGAACCGGCATGTCGT758290.16416809831233273No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGCGGCATGTCGTC545820.11816881591585997No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGCCTTGGCCG537590.11638703922210097No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGCAG512800.11102005936325708No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCGCCTT505430.10942447075657379No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)