FastQCFastQC Report
Fri 30 Jun 2023
SRR1964314.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1964314.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21259213
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAG215022910.114339604198895No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCA5696952.679755831036643No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGCA2203011.036261314094741No Hit
CCTCCCGGGGCTCATAAGTGAGTCTATGATAGATAAGGTTAATGCACTAC1651890.7770231193412475No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGA1373730.6461810227876262No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGC1305970.6143077827010812No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGAC1121730.5276441794905578No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAGC937660.44106054161083014No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAG654640.3079323773650511No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCTTGGCCGTACAGCAGACCA593410.27913074674965627No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCGCCTT478190.2249330678421633No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCCGCCTTGGCCGTACAGCAG470230.2211888088237321No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACGCCTTGGCCGTACAGC461260.21696946166351502No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGCCTTGGCCG370010.1740468943982075No Hit
CCTCCCGGGGCTCATAAGTGAGTCTATGATAGGATAAGGTTAATGCACTA357340.16808712533243822No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCGCCTTGGCCGTACAGCA336890.15846776642202137No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCCTTGGCCGTA334900.15753170166741354No Hit
CTCCCGGGGCTACGCCTCAGACTCGCAGCGAGCGGAACCGGCATGTCGTC321750.1513461481382213No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCCGCCTTGGCCGT272360.12811386762059349No Hit
CTCCCGGGGCTACGCCTGTCTGATATGATAGATAAGGTTAATGCACTACT272170.12802449460382187No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGCAG264250.12429905095734257No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCGCCTTGGCCGTACA258450.12157082202431482No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCCGCCTTGGCCGTACAGCA258260.12148144900754324No Hit
GTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACA250490.11782656300588362No Hit
CTCCCGGCCGATGCGGACAGACTCGCAGCGAGCGGAACCGGCATGTCGTC240910.11332028142339982No Hit
CTCCCGGGCGATGCGGACAGACTCGCAGCGAGCGGAACCGGCATGTCGTC236910.11143874422820826No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCGCCTTGGCCGTACAGCAGAC220450.10369621866999498No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCGCCT213580.10046467853725347No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)