FastQCFastQC Report
Mon 10 Jul 2023
SRR1964315.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1964315.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43321863
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCCATGTCGTC8081521.865459941092561No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAG7283041.6811465379501338No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCA5373331.2403275454705167No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGC1646920.38015908964949174No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCCATGTATGA1304930.30121742455997336No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTCAATTTGCACTACT963870.22249043167880383No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACGCCTTGGCCGTACAGC867450.20023377110998203No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCGCCTTGGCCGTACAGCAGCT821490.18962480907157664No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGC792340.1828961049066611No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCTTGGCCGTACAGCAGCTTA697310.1609602984987049No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCCTACAGCAG691060.15951760892646744No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCTTGGCCGTACAGCAGCTT655660.15134621518931446No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGCT653250.15078991409025969No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCTGCACTACT625460.14437513917626305No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN522440.12059499841915848No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACGATG514660.11879913843963728No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGCA506440.11690171311423056No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCCGCACTACT482600.11139871800988799No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACTCGT454850.10499317630915364No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)