FastQCFastQC Report
Sat 1 Jul 2023
SRR1964320.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1964320.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30423239
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAG11232393.6920427834787746No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCA2171050.7136156672864451No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAGC2126250.6988900820192091No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAG1410190.4635239528572221No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGCA1378320.4530484081593022No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCGCCTT1220570.4011965984292468No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACGCCTTGGCCGTACAGC1054130.3464884195926673No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCTTGGCCGTACAGCAGTATA1029170.33828416494377866No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGCCTTGGCCG978380.32158969003924925No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCCTTGGCCGTA947640.31148557193400744No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGT940840.3092504384559448No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGC780410.256517723178653No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGTA581300.19107104276438153No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCGCCTTGGCCGTACA575380.1891251618540682No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCCGCCTTGGCCGT569310.18712997652879762No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCGCCTTGGCCGTACAGCAGTA526370.17301576600703167No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCGCCT498280.1637826925660348No Hit
GTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACA487900.16037082705099218No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCCTTGG467930.15380676593968184No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCCGCCTTGGCCGTACAGCA460310.15130210165985286No Hit
GTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCCTTGGCCGTAC393670.12939779357483927No Hit
CCTCCCGGGGCTCATAAGTGAGTCTATGATAGATAAGGTTAATGCACTAC374000.12293234129344348No Hit
GTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGCCTTGGCCGT369270.1213776087417911No Hit
AATCAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGCGCCTTGGCCGTAC327990.10780903374555222No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCGCCTTGGCCGTAC311940.10253346134512502No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)