FastQCFastQC Report
Sun 2 Jul 2023
SRR1964321.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1964321.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50476436
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAG28056975.558429283715673No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCA9415101.8652465875364101No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGCA2998670.5940732424135491No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGT2743350.5434912243011769No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGC2477620.4908468577298128No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGTG1815520.35967674104407843No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAGC1613470.31964816216422254No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCGCCTTGGCCGTACAGCAGTG1391670.2757068664673552No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCTTGGCCGTACAGCAGTGTC1371600.271730753732296No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGCCTTGGCCG1218160.2413324110283856No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCCTTGGCCGTA1015890.2012602474548718No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCGCCTT906570.17960261695179905No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAG901220.17854271644693773No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACGCCTTGGCCGTACAGC802450.1589751701170027No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCTTGGCCGTACAGCAGTGT675090.13374359473398637No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN531740.10534420457102003No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)