FastQCFastQC Report
Sun 2 Jul 2023
SRR1964323.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1964323.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25168492
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAG18187867.2264401061454135No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCA4041381.6057298943456761No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGCA2042090.8113676417323692No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCTTGGCCGTACAGCAGTGTC1508360.599304876907206No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGT1144220.45462397985544784No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGC1141470.4535313438723305No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGTG1005690.3995829388586333No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAGC995380.3954865472273825No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCGCCTT995110.39537927023994923No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCCTTGGCCGTA875650.34791516313333354No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGCCTTGGCCG865200.3437631463974878No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACGCCTTGGCCGTACAGC817870.32495788782259977No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAG752650.29904453552481414No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCGCCTTGGCCGTACAGCAGTG719180.28574616230483735No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCCGCCTTGGCCGT497310.19759229118693325No Hit
CCTCCCGGGGCTCATAAGTGAGTCTATGATAGATAAGGTTAATGCACTAC457720.18186230625180086No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCGCCTTGGCCGTACAGCA434490.17263251211077724No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCGCCT419980.16686736734167468No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCCGCCTTGGCCGTACAGCAG395820.15726806357726955No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCGCCTTGGCCGTACA395350.15708132215470041No Hit
CGCGACCTCAGATCAGACGTGGCGACCCTAGTCATAAGGTTAATGCACTA365690.14529674642406068No Hit
CTCCCGGGGCTACGCCTGTGACTCGCAGCGAGCGGAACCGGCATGTCGTC362790.14414451211459153No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCCTTGG327580.13015479830893326No Hit
GTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACA327500.13012301253487893No Hit
GTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCCTTGGCCGTAC314150.12481876148956403No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGTCTATTCCAATTACGTGATGA307460.12216067613427138No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCGCCTTGGCCGTAC293660.1166776301099009No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCCGCCTTGGCCGTACAGCA293090.11645115646976387No Hit
GTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGCCTTGGCCGT292470.11620481672084287No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCTTGGCCGTACAGCAGTGT271220.1077617204876637No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)