FastQCFastQC Report
Thu 6 Jul 2023
SRR1964325.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1964325.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24502833
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAG14121255.763109106608203No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCA4497111.8353428764747326No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGCA1582740.6459416345856824No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGC1421610.5801818916204505No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGGG1325280.5408680702349806No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAGC1097220.4477931184528744No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGG996690.40676520955760503No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACGCCTTGGCCGTACAGC907290.370279632563304No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCTTGGCCGTACAGCAGGGCA850320.3470292598410967No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAG805260.328639549557392No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCGCCTTGGCCGTACAGCA653730.26679772090027304No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCGCCTT625520.2552847664594539No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCCTTGGCCGTA532570.21735037740329863No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCCTTGG413160.1686172370354073No Hit
GTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACA409710.16720923658092923No Hit
CTCCCGCCCGATGCGGACAGACTCGCAGCGAGCGGAACCGGCATGTCGTC399080.16287096271684176No Hit
CTCCCGAAATCGTATTCACTCAGATACTATCTATTCCAATTACGTGATGA390990.15956930368010916No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCGCCTTGGCCGTACA343020.1399919756217577No Hit
CTCCCGTTTAGCATAAGTGAGTCTATGATAGATAAGGTTAATGCACTACT304010.12407136758431157No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGCCTTGGCCG289000.11794554531714761No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCGCCTTGGCCGTACAGCAGGG278310.11358278448863443No Hit
ATCAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGCGCCTTGGCCGTACA274160.11188910278252313No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGCGCCTTGGCCGTACAG259670.10597550087371531No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCTTGGCCGTACAGCAGGGC255120.10411857273809931No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCCGCCTTGGCCGTACAGCA249190.10169844442069209No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCGCCTTGGCCGTA248420.10138419504389554No Hit
AATCAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGCGCCTTGGCCGTAC245420.10015984682261027No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)