FastQCFastQC Report
Fri 30 Jun 2023
SRR1964326.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1964326.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27538483
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAG21600117.843609250371562No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCA3773351.3702098260096607No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACGCCTTGGCCGTACAGC2494310.9057543220517992No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGCA2217970.8054074728807684No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCTTGGCCGTACAGCAGGGCA1334240.48450018107388126No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGGG1262740.4585365141572977No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGCCTTGGCCGTACAGC1059490.3847307057545617No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAGG1002810.3641485988897791No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAGC975430.35420614853766635No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCGCCTT869560.3157617650906914No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAG834550.3030486465067811No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCCGCCTTGGCCGTACAGCAG744740.27043610209030033No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCCTTGGCCGTA721280.2619171143159919No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCGCCTTGGCCGTACAGCA702600.2551338793789041No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGCCTTGGCCG622010.2258693770459324No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCGCCTTGGCCGTACAGCAGGG593010.21533865899584953No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCGCCTTGGCCGTACA546980.1986238675529077No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGTCTATTCCAATTACGTGATGA469190.17037612420408196No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCCTTGG462780.16804847238680504No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCCGCCTTGGCCGT447160.16237640976810525No Hit
GTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACA419720.1524121717234751No Hit
CTCCCGGGGCTACGCCTGTGACTCGCAGCGAGCGGAACCGGCATGTCGTC360810.13102028895346196No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACGCCTTGGCCGTACAGCA353710.12844207867223478No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCGCCTTGGCCGTAC353380.12832224636338901No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACGCCTTGGGGCATGTCG339950.12344543452157478No Hit
ATCAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGCGCCTTGGCCGTACA336260.12210549143175388No Hit
GTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCCTTGGCCGTAC312310.11340857083521994No Hit
AATCAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGCGCCTTGGCCGTAC303410.11017672977847036No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCGCCT286770.10413427638697455No Hit
CAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGCCTTGGCCGTACAGC285360.10362226561281535No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACGCCTTGGCCGTACAG280550.1018756189293361No Hit
CTCCCGGCCGATGCGGACAGACTCGCAGCGAGCGGAACCGGCATGTCGTC276600.10044126250527308No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)