FastQCFastQC Report
Sun 2 Jul 2023
SRR1964327.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1964327.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34155138
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGCCTTGGCCGTACAGCAG1149390.336520379452134No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAGC853510.2498921245758105No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACGCCTTGGCCGTACAGC570660.16707881549183023No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGCCTTGGCCGTACAG550070.1610504399074599No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCGCCTTGGCCGTACA464090.13587706774892844No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGCGCCTT405650.11876690411849603No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN370190.10838486437970182No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCCTTGGCCGTA357410.10464311401698917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)