FastQCFastQC Report
Sat 23 Sept 2023
SRR19749671_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19749671_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94473456
Total Bases4.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGTTGAACCCCATTCGTGATGGGGATCGGGGATTCAACTGGAATTCT1897500.20085006734590086No Hit
CCCGGTTGAACCCCATTCGTGATGGGGATCGGGGATTCAACTGGAATTCT1748090.1850350430707224No Hit
CCCGGTTGAACCCCATTCGTGATGGGGATCGGGGATTCATCTGGAATTCT1727090.18281219647558994No Hit
CCCTGTTGAACCCCATTCGTGATGGGGATCGGGGATTCAACTGGAATTCT1663740.17610660924694022No Hit
CCCTGTTGAACCCCATTCGTGATGGGGATCGGGGATTCATCTGGAATTCT1622020.171690554011277No Hit
CACGAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGTCCTGGAAT1617420.17120364475710512No Hit
CCCAGTTGAACCCCATTCGTGATGGGGATCGGGGATTCATCTGGAATTCT1529380.16188462503160675No Hit
CCCAGTTGAACCCCATTCGTGATGGGGATCGGGGATTTAACTGGAATTCT1451750.15366750211826694No Hit
CCCTGTTGAACCCCATTCGTGATGGGGATCGGGGATTTAACTGGAATTCT1394600.14761818388437065No Hit
CCCGGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTACTGGAATTCT1269640.13439118814495365No Hit
CCCTGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTACTGGAATTCT1222100.12935908685292513No Hit
CCCAGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTACTGGAATTCT1207800.12784543417147776No Hit
CCACGGCCGAGGTGGGATCCCGTGCCTGGAATTCTCGGGTGCCAAGGAAC1179880.12489010669833017RNA PCR Primer, Index 1 (100% over 24bp)
CCCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTCATCTGGAATTCT1174690.12434074603981884No Hit
CCCGGTTGAACCCCATTCGTGATGGGGATCGGGGATTTAACTGGAATTCT1159400.12272230201888666No Hit
CCCGGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTTCTGGAATTCT1136510.12029939923019223No Hit
CCCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTCAACTGGAATTCT1117580.11829566179943707No Hit
CCCTGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTTCTGGAATTCT1075020.11379069269996855No Hit
CCCGGTTGAACCCCATTCGTGATGGGGATCGGGGATTCGACTGGAATTCT1032510.10929101609239318No Hit
CCCTGTTGAACCCCATTCGTGATGGGGATCGGGGATTCAGCTGGAATTCT1010350.10694538368533908No Hit
CCCGGTTGAACCCCATTCGTGATGGGGATCGGGGATTCAGCTGGAATTCT1005980.10648281989387579No Hit
CCCAGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTTCTGGAATTCT1000640.10591758175968496No Hit
CCCTGTTGAACCCCATTCGTGATGGGGATCGGGGATTCGACTGGAATTCT990420.10483579641672049No Hit
CCACGGCCGAGGTGGGATCCCGGGCCTGGAATTCTCGGGTGCCAAGGAAC986620.10443356703283936RNA PCR Primer, Index 1 (100% over 24bp)
CCCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTTAACTGGAATTCT976040.10331367574824403No Hit
CCCAGTTGAACCCCATTCGTGATGGGGATCGGGGATTCAGCTGGAATTCT954310.10101355877147121No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)