FastQCFastQC Report
Sat 23 Sept 2023
SRR19754255.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19754255.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21331756
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGAACCGTGGAAGACGACCACGTTGATAGGCCGGGTGTGGAAGTGCGG694503432.55725407697332No Hit
TTGAGAACCGTGGAAGACGACCACGTTGATAGGCCGGGTGTGGAAGTGCG12309305.770411024765144No Hit
GTCATAGCTGTTTCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4093051.9187590557476844Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAGAACCGTGGAAGACGACCACGTTGATAGGCCGGGTGTGGAAGTGCGGC3387001.587773646013952No Hit
AAGCTTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTCTGAACT2493321.1688301703807227Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTCTGAACTC1584920.7429861845410195Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTA1529500.7170061386413757TruSeq Adapter, Index 19 (97% over 38bp)
GTGAGAACCGTGGAAGACGACCACGTTGATAGGCCGGGTGTGGAAGTGCG1371910.6431303639512846No Hit
TTACGTTGGTTCAAATCCAACTCCCTCCACCAAGATCGGAAGAGCACACG1173260.5500062910901475No Hit
CTGAGAACCGTGGAAGACGACCACGTTGATAGGCCGGGTGTGGAAGTGCG1020010.47816504182778014No Hit
TAAGGGTCTTTGCGCCGAAAATGTAACGGGGCTGAAGCCATGCACCGAAG903140.42337817852407467No Hit
GCGGGTGTAGCTCAGGGGTAGAGCACAACCTTGCCAAGGTTGGGGTCGGG891740.4180340333913439No Hit
ATCGCGGATACGAGAGATCGTAGATCGGAAGAGCACACGTCTGAACTCCA731440.3428878522705773Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCATGAAGCTTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTC703420.329752506075918No Hit
GCGGGTGTAGCTCAGTCGGTTAGAGTGCCGGCCTGTCACGCCGGAGGTCG677300.31750785073671384No Hit
TGAAGCTTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTCTGAA639960.30000343150371683Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAAGAAGGGCATTTGGTGGATGCCTTGGCATGCAGATCGGAAGAGCACA587170.2752562892618873No Hit
CGACCTGGTGGTTCTGGCGGGGTGGCTGCACCCGTTCCCATTCCGAACAC509410.23880359404073437No Hit
GCATGAAGCTTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTCT483680.2267417647192289Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTCTGAACTCC471350.22096164985198594Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGAAGCTTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTCTGA467160.218997442123377Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTA461940.21655038619417924TruSeq Adapter, Index 19 (97% over 38bp)
CATGAAGCTTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTCTG438540.20558082513225823Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGGTCTTTGCGCCGAAAATGTAACGGGGCTGAAGCCATGCACCGAAGC433680.20330253168093618No Hit
CTACGTTGGTTCAAATCCAACTCCCTCCACCAAGATCGGAAGAGCACACG416090.19505660949806478No Hit
ATGAAGCTTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTCTGA403360.18908898076651542Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCATGGCATGCAAGAGGTCAG355020.16642793026509398No Hit
TACGTTGGTTCAAATCCAACTCCCTCCACCAAGATCGGAAGAGCACACGT349720.16394337156303496No Hit
TGGGAACCGTGGAAGACGACCACGTTGATAGGCCGGGTGTGGAAGTGCGG331250.1552849188786896No Hit
GGGCGTGTAGCTCAGCGGGAGAGCACTACGTTGACATCGTAGGGGTCACA323930.15185341516188353No Hit
CCTACGTTGGTTCAAATCCAACTCCCTCCACCAAGATCGGAAGAGCACAC319960.1499923400586431No Hit
GGGGCTGTAGCTCAGCTGGGAGAGCACCTGCTTTGCAAGCAGGGGGTCAG308570.14465288277251998No Hit
TTAAGGGTCTTTGCGCCGAAAATGTAACGGGGCTGAAGCCATGCACCGAA301990.14156827970468067No Hit
TAATCGTTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAG301210.1412026276692833TruSeq Adapter, Index 19 (97% over 38bp)
TGTGAACCGTGGAAGACGACCACGTTGATAGGCCGGGTGTGGAAGTGCGG278130.13038307769880736No Hit
ATGAGAACCGTGGAAGACGACCACGTTGATAGGCCGGGTGTGGAAGTGCG272030.12752349126813564No Hit
TCTACGTTGGTTCAAATCCAACTCCCTCCACCAAGATCGGAAGAGCACAC248070.11629141079618574No Hit
TAATCTCTTGCTTCCTGGCACGAGAGATCGGAAGAGCACACGTCTGAACT228120.10693915681390692Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAAGCTTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTCTGAAC222610.10435615333308706Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACCCGTAGCTCAGCTGGATAGAGCACCAGACTGCGAATCTGGGGGTCA220450.10334357846583282No Hit
TTTCTGGCGGGGTGGCTGCACCCGTTCCCATTCCGAACACGGCCGTGAAA219900.1030857469024116No Hit
GTTCTGGCGGGGTGGCTGCACCCGTTCCCATTCCGAACACGGCCGTGAAA217500.10196066371657354No Hit
AGGAGGCAGCTAACCACGGTAGGGTCAGCGACTGGGGTGAAGTCGTAACA216090.10129967734489367No Hit
GGTGGTTCTGGCGGGGTGGCTGCACCCGTTCCCATTCCGAACACGGCCGT214970.10077463852483594No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)