FastQCFastQC Report
Sat 23 Sept 2023
SRR19754257.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19754257.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17510178
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGAACCGTGGAAGACGACCACGTTGATAGGCCGGGTGTGGAAGTGCGG257043414.679656597437216No Hit
GTCATAGCTGTTTCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4337022.476856603056805Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGAGAACCGTGGAAGACGACCACGTTGATAGGCCGGGTGTGGAAGTGCG3428651.9580897464320464No Hit
AAGCTTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTCTGAACT3126601.785590072242555Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTACGTTGGTTCAAATCCAACTCCCTCCACCAAGATCGGAAGAGCACACG2036151.1628379791456147No Hit
AGCTTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTCTGAACTC1725400.9853697660869011Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA1318690.7530991403970879TruSeq Adapter, Index 2 (97% over 37bp)
GAGAACCGTGGAAGACGACCACGTTGATAGGCCGGGTGTGGAAGTGCGGC1189090.6790850441383292No Hit
CGCATGAAGCTTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTC1183630.6759668576755758No Hit
TGAAGCTTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTCTGAA1120780.6400734475686084Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTACGTTGGTTCAAATCCAACTCCCTCCACCAAGATCGGAAGAGCACACG1033070.5899825804169437No Hit
TACGTTGGTTCAAATCCAACTCCCTCCACCAAGATCGGAAGAGCACACGT995520.5685379097802433No Hit
GCATGAAGCTTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTCT960640.5486180665896143Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGAAGCTTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTCTGA956510.5462594383677881Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CATGAAGCTTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTCTG851510.48629431408407153Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAAGCTTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTCTGA747640.4269745287569321Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGGTGTAGCTCAGTCGGTTAGAGTGCCGGCCTGTCACGCCGGAGGTCG682440.38973904205885285No Hit
GCTTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTCTGAACTCC615140.35130425287509925Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTACGTTGGTTCAAATCCAACTCCCTCCACCAAGATCGGAAGAGCACAC582090.33242951613627225No Hit
AGTGCTGGTTCGACTCCGGCATGGGGCACCAAGATCGGAAGAGCACACGT479150.27364085048136005No Hit
ATCGAGGGTTCGAATCCCTTCTTCTCCGCCAAGATCGGAAGAGCACACGT455740.2602714832482No Hit
TCTACGTTGGTTCAAATCCAACTCCCTCCACCAAGATCGGAAGAGCACAC451090.257615884887064No Hit
AAAAGAAGGGCATTTGGTGGATGCCTTGGCATGCAGATCGGAAGAGCACA443660.2533726384734638No Hit
TTTCCCTGGTTCGAATCCAGGCGCCCCAGCCAAGATCGGAAGAGCACACG429180.24510316228652843No Hit
ACGTTGGTTCAAATCCAACTCCCTCCACCAAGATCGGAAGAGCACACGTC399630.22822726302382537No Hit
GAAGCTTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTCTGAAC370320.21148842690234215Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGAGAACCGTGGAAGACGACCACGTTGATAGGCCGGGTGTGGAAGTGCG352650.20139715313002526No Hit
TAAGGGTCTTTGCGCCGAAAATGTAACGGGGCTGAAGCCATGCACCGAAG304040.17363615606877325No Hit
TGTGGAGGTTCGAGTCCTCTTCTGGGCACCAAGATCGGAAGAGCACACGT284350.16239126752452204No Hit
CTGAGAACCGTGGAAGACGACCACGTTGATAGGCCGGGTGTGGAAGTGCG278460.1590275096004164No Hit
ACGCATGAAGCTTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGT265640.15170605347358548No Hit
GGTGTGGAAGTGCGGCAACGCATGAAGCTTACCGGTACTAATCGTTCGAG257110.1468346009960607No Hit
TAATCTCTTGCTTCCTGGCACGAGAGATCGGAAGAGCACACGTCTGAACT247890.14156909198752862Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAGAAGGGCATTTGGTGGATGCCTTGGCATGCAGATCGGAAGAGCACAC245530.14022130443219938No Hit
TTACGCCGGTTCAATTCCGGCCGAGGCCTCCAAGATCGGAAGAGCACACG244970.13990149043601954No Hit
AAAATCCAACTCCCTCCACCAAGATCGGAAGAGCACACGTCTGAACTCCA241310.13781127753241573Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAGCTTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTCTGAA227840.1301186087314475Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACGTTGGTTCAAATCCAACTCCCTCCACCAAGATCGGAAGAGCACACG216680.12374517266472106No Hit
TCGTTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTA211160.12059272041666282TruSeq Adapter, Index 2 (97% over 37bp)
TACGCCGGTTCAATTCCGGCCGAGGCCTCCAAGATCGGAAGAGCACACGT210200.12004446785178313No Hit
GCGGGTGTAGCTCAGGGGTAGAGCACAACCTTGCCAAGGTTGGGGTCGGG207480.11849108558462397No Hit
TACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTCTGAACTCCAGT206580.11797709880504927Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGAAGTGCGGCAACGCATGAAGCTTACCGGTACTAATCGTTCGAGATCG196460.1121976030169425No Hit
TTACGTTGGTTCAAATCCAACTCCCTCCACCAGATCGGAAGAGCACACGT194180.11089550317535321No Hit
TTTGATAGGCCGGGTGTGGAAGTGCGGCAACGCATGAAGCTTACCGGTAC192000.1096505129759389No Hit
TTACCGGTACTAATCGTTCGAGATCGGAAGAGCACACGTCTGAACTCCAG184190.10519024992207389Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGGGTCTTTGCGCCGAAAATGTAACGGGGCTGAAGCCATGCACCGAAGC182560.1042593627546219No Hit
AGACGTGTGGTAGCGGAGCGTTCCGTAAGCCAGTGAAGGGATACCCGTGA177430.10132963811104603No Hit
GCCTACGTTGGTTCAAATCCAACTCCCTCCACCAAGATCGGAAGAGCACA177290.1012496846120011No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)