FastQCFastQC Report
Thu 6 Jul 2023
SRR1976443.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1976443.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191722467
Total Bases10.7 Gbp
Sequences flagged as poor quality0
Sequence length51-58
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGT77395944.036873779638982No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT49652842.5898289739824807No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA42319622.2073375469344443No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC34406101.7945784100514415No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG31575641.6469452169108592No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT27896111.4550256126216026No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC24838041.295520571410131No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT23750291.2387849150720558No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCG22426751.1697507522683817No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATTCGTA20778371.0837733482743053No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTAT15247820.7953068953572353No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGT13116260.6841274371876302No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT13114470.6840340730645824No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGT11439590.596674462779576No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA11117160.5798569241235562Illumina Single End Adapter 1 (95% over 22bp)
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTA10404590.5426901793413705No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATTCGTA9775960.5099016381841154No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC9488470.49490652548300457No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATT9274110.4837257805575807No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTC8465450.4415471036057553No Hit
GAGGGGCCCGTGCCTTGGAAAGCGTCGCGCTGTAGGCACCATCAATTCGT6634900.34606794413927505No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAAT6189250.3228234070240709No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT6043280.31520979750380534No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCCTGTAGGCACCATCAATTCGT6003780.31314952774940036No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTATGC5922620.30891632538819774No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTATG5505000.28713379741770173No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT5279040.2753480112479462No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCG4925000.2568817352011228No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTC4872580.2541475746814795No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTCG4787220.2496953056628465No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGCTGTAGGCACCATCAATTC4559090.23779633505341866No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCCTGTAGGCACCATCAATTCG4450350.2321245949750897No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATTC4318880.22526728701023863No Hit
GGGGCCACGCGCGCGTCCCCCCTGTAGGCACCATCAATTCGTATGCCGTC4196230.21887001902599137No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTCG4165460.21726509496667387No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT4149650.2164404654776323No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATTCG3754990.19585550190108914No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTA3590380.1872696536916563No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATTC3526470.18393618938775702No Hit
GACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATG3432230.17902075086484256No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCCTGTAGGCACCATCAATTCGT3407330.17772199853864806No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATT3227490.1683417729023902No Hit
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGTATGC3163870.16502343462959926No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATTC3151540.16438031751385715No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCGT3149100.16425305021763567No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG3040290.15857765902834955No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGTATG2895520.15102663998163554No Hit
CTCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATT2854210.14887196292962368No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGCTGTAGGCACCATCAATTCG2813390.14674284365431203No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTCTGTAGGCACCATCAATTCGTAT2774240.14470082945469295No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATTCGTA2773720.14467370691615397No Hit
CGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTAT2731290.14246061208882732No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTAT2723060.14203134575771967No Hit
AGAGGGGCCCGTGCCTTGGAAAGCGTCGCGCTGTAGGCACCATCAATTCG2692460.14043528868215535No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATT2673520.13944740237460015No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATTCG2671200.13932639412573383No Hit
CGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAA2604490.13584688538354767No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCCTCAATTCGT2580390.13458986003972087No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGACTGTAGGCACCATCAATT2508210.13082504305559556No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATTCGT2492120.1299858091227253No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT2451080.12784521492726253No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC2409990.1257020127954018No Hit
TCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCCTGTAGGCACCATCAATTC2399880.12517468805573007No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCCTGTAGGCACCATCAATTC2367530.12348735320623638No Hit
GACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGTAT2360760.12313423861794977No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT2345710.12234924976215751No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCCTGTAGGCACCATCAATT2335360.12180940692777543No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATT2300660.11999949906757668No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATTC2271670.11848741754402732No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT2222130.11590347416090782No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATTCG2185000.11396682059176715No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGTATGCCGTCT2156400.11247508097212207No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT2148130.11204372829189602No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCACCATCAATTC2049100.10687844946205495No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAATT2039800.10639337329203051No Hit
GCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATTCGTA1997690.10419696925765096No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT1971670.10283979915614166No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATT1953620.10189833411647056No Hit
GGGGCCACGCGCGCGTCCCCCTGTAGGCACCATCAATTCGTATGCCGTCT1928370.10058132623549018No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA1922950.10029862592994905No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)