FastQCFastQC Report
Tue 4 Jul 2023
SRR1976445.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1976445.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149434585
Total Bases7.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT84030215.623210316407008No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT67106804.490714114138973No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT59783414.000640815511349No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC25965161.7375602843210627No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG23793881.5922605867978956No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC22726931.5208614525211817No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT20833961.3941859576884426No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC18188791.2171740564608922No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT17599511.1777400793798838No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT11263470.753739169550342No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGT10429640.6979401722834109No Hit
CGTTGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT10391820.6954092989919302No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC10239540.6852188869129593No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT10032920.6713921010989524No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT10023030.6707302730489063No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT9951200.6659234875246584No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA9558320.6396323849663048No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA9308590.6229207248107926No Hit
CTTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT9147400.6121340652165628No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC8887750.594758569443613No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT7573040.5067796052700919No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT7379320.4938160734344061No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA7361240.49260617948649577No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA7359520.49249107895605293No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGT6753440.4519328641358357No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG5886980.39395030273614373No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTC5708850.3820300367548784No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAAT5671330.37951923913731217No Hit
CGTGTGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT5519080.36933083462573274No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA5485140.3670596067168788No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC5243800.35090939624184053No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT5154760.3449509362240341No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATT4765680.3189141255352635No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT4366490.2922007646355762No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTA4361540.2918695160159879No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA4296450.2875137639656844No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT4048430.2709165351514845No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCG3921290.2624084645465439No Hit
GTGTGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC3750910.2510068201414017No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA3523530.23579079769251543No Hit
TTTTTGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC3452700.2310509310813156No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA3421400.2289563691029088No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA3254060.21775815819343294No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGCTGTAGGCACCATC2932250.19622298278541075No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT2828690.1892928601501453No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATTC2809140.18798459540005416No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA2772550.18553603237162267No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGCTGTAGGCACCAT2766400.1851244810563766No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2744020.18362683578235922Illumina Single End Adapter 1 (95% over 22bp)
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCGT2600440.17401861824690718No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGCTGTAGGCACCAT2582890.1728441913229123No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTAT2571040.17205120220329181No Hit
TCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT2557420.17113976660757615No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC2515700.16834790955520773No Hit
TCGTTGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2499920.16729192910730806No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTAT2478300.16584514220720725No Hit
CTTTTGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2405290.1609593923655625No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATT2400100.16061208320684264No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGCTGTAGGCACCA2318770.15516956800863735No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT2171530.14531642725142913No Hit
TCTGCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT2126870.14232782859469914No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA2099400.1404895660532667No Hit
TCGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2081830.13931380075101088No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT2008780.1344253741528442No Hit
TCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGT1967400.13165626953091214No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT1934940.1294840816133695No Hit
GTGGTGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC1928710.12906717678507956No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATT1922060.12862216601330945No Hit
TCTCGCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT1886780.12626126676097105No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT1886350.1262324916283603No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1867310.12495835552392373No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA1855650.12417808099778241No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGT1825390.12215311468894566No Hit
CCTGCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT1824870.12211831685416062No Hit
CCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1811500.12122361098670699No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTCG1793720.12003379271271104No Hit
CCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG1705630.11413890566230034No Hit
GCCTCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1691610.1132007025013654No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATT1666610.11152772967516188No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGT1647290.11023485627507179No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAA1584200.1060129420508646No Hit
CCTCGCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT1504050.10064939117005611No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1501860.10050283875048069No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)