FastQCFastQC Report
Mon 10 Jul 2023
SRR1976447.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1976447.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205566796
Total Bases11.5 Gbp
Sequences flagged as poor quality0
Sequence length51-58
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT75599263.6776007347023105No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC64296153.1277497753090437No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGT60377382.9371173348442907No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC42462392.065624936821022No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT41201672.0042959661637183No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC32973391.604023151676694No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT27675041.346279678358172No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA26224561.2757196449177521No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTAT25274471.2295015776769707No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA25214931.2266051955200004Illumina Single End Adapter 1 (95% over 22bp)
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCG22957891.116809253572255No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATTCGTA20715621.0077318128750714No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT18672360.9083354103548902No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTC18648850.9071917431645916No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGACTGTAGGCACCATCAATT16465430.8009771188922942No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATTCGTA16388580.7972386746739002No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG12477970.6070031854755376No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGCTGTAGGCACCATCAATTC12217950.5943542555384285No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTCG11908510.5793012408482545No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGT11481530.5585303766664729No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTC11296010.5495055728747167No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTCG8571150.41695206457369705No Hit
GAGGGGCCCGTGCCTTGGAAAGCGTCGCGCTGTAGGCACCATCAATTCGT8450030.41106006244315835No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA8372460.407286593113024No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATT8234760.4005880404926873No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT8117610.3948891629366058No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATT8106370.39434238202554855No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTA7641260.3717166463011857No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATTC7372690.3586517931621603No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT6686000.32524707929971336No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCCTGTAGGCACCATCAATTCG6569680.3195885779141102No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA6037520.29370112865892994No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT5534260.2692195484722153No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCCTGTAGGCACCATCAATTCGT5426870.2639954557641692No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATTCG5338010.25967277322355115No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATT5174350.2517113707410218No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAATT5147980.2504285760235325No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAAT5130630.24958456812256782No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTAT5013850.24390368958224165No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTCG4940160.24031896668759678No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT4675710.2274545350213076No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGT4602480.22389218928138568No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTATG4588830.22322817153797542No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT4533220.22052296811592084No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTCTGTAGGCACCATCAATTCGTAT4375550.21285295510467556No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC4130750.20094441711296604No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTATGC4036580.1963634243732631No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTCTGTAGGCACCATCAATTCGTAT3972280.1932354873108982No Hit
GCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATTCGTA3936790.1915090411780315No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT3817300.18569633200879387No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCCTGTAGGCACCATCAATTCGT3731280.18151180407559594No Hit
CGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTAT3691160.17956012701584356No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG3683780.17920111962050525No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATTCGT3381460.16449446436865223No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATTC3358810.16339263272848792No Hit
AGAGGGGCCCGTGCCTTGGAAAGCGTCGCGCTGTAGGCACCATCAATTCG3277990.15946106393563678No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT3230380.15714502842180797No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATTCG3136120.15255965754313747No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTCG3073930.14953436351656715No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT2906270.1413783770799249Illumina Single End Adapter 1 (95% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATG2844360.1383667039301425No Hit
CTTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT2806780.1365385876812518No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGTATG2711690.13191284063210287No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAAT2662660.12952772781456398No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT2660790.1294367598160162No Hit
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGTATGC2647360.12878344419008214No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCTGTAGGCACCATCAATT2639980.12842443679474383No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATTCGT2566390.12484457849895175No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT2563740.12471566662935193No Hit
GGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATTC2483800.12082690630640562No Hit
CGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTATG2461770.11975523517912884No Hit
GGCGGAGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATTCG2385510.11604549209396638No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATTCG2347440.11419353931069685No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCCTCAATTCGT2334810.11357914047558537No Hit
GTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATTCG2314680.1125998967265122No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATT2310970.11241942010907248No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT2309160.1123313708698364No Hit
GGCGGAGCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATTC2263440.11010727627432594No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATT2194230.10674048740828748No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATTC2146430.10441520915663832No Hit
CTCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATT2118230.10304339228014237No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAATTC2073420.10086356553419261No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATG2060760.10024770731942526No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG2060610.10024041042114604No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)