FastQCFastQC Report
Sun 2 Jul 2023
SRR1976448.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1976448.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131095287
Total Bases6.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT105464488.044871971636935No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT69417355.29518273223659No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT65319094.982565849220804No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC32427832.473607613369045No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC31096882.3720822244357267No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT24288351.8527248809486185No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT21199541.6171092405480603No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT16900291.28916076136284No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC16757821.2782930937860488No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA14914881.137712906490681No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA11607820.8854490703391954No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA11047410.8427007753528165No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT10573950.8065850605292926No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC10517000.8022408921535067No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT10486510.7999151029739155No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT10069720.7681221980161652No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG8831860.673697750858122No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT7987940.6093232016800115No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGT7559740.576659937439246No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT7116290.5428333972067203No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT7066720.5390521781305532No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA6798730.5186097956366654No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC6536640.49861746746090113No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGT6504290.49614979675051174No Hit
CTTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT6409410.48891231307194133No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTC6403470.4884592075381017No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAAT5969500.4553558054302898No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA5968270.45526198054702No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT5390500.4111894579398571No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA5369910.40961884464999876No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT4874090.3717975002411795No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA4812480.36709786523446875No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC4735020.3611891860002564No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATT4680680.35704410944994536No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTAT4506400.34374996257493223No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA4431650.33804800320548517No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA4263630.32523137159004045No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG3974610.3031848124334173No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATT3885320.2963737361511707No Hit
CGTTGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT3794740.2894642581620802No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATTC3592570.27404265112902193No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCG3573670.2726009517031684No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3524160.26882430945057545Illumina Single End Adapter 1 (95% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA3517180.26829187230811735No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA3233000.2466145102531413No Hit
CGTGTGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT3198960.24401792567874694No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGT3093220.2359520369332576No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT2962480.22597913836521064No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT2874470.21926570098587905No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC2620770.19991336530656512No Hit
TCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT2612470.19928023804547604No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATTCGT2500870.19076734619757918No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT2481410.18928292975170039No Hit
CCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2418980.1845207448228097No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGCTGTAGGCACCATC2377020.1813200195366291No Hit
AGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATTCGTATGCCG2259590.17236241299811184No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA2209340.16852932325477116No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG2204400.1681524981138338No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA2154630.16435602295908625No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGCTCGCACCTGTAGGCACCATC2126960.16224534448747954No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2092440.1596121453244921No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATT2076900.15842674801879034No Hit
TTTTTGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2059230.15707887347620667No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTAT2035400.1552611117133448No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAAT1971710.1504028134893972No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTCG1906000.14539042887178696No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT1902780.14514480600664156No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATT1862010.14203485438801472No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTA1827090.13937114306786635No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAGGCACCATCAA1784260.1361040538398608No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGT1767650.13483703651375353No Hit
GTGTGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC1764730.13461429776647882No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATT1764290.1345807343936018No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGCTGTAGGCACCAT1741020.13280568965076525No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA1733350.13222061903720458No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATTCGTA1704620.13002908334912147No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATG1629040.12426381125356552No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG1609540.12277634359197064No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCG1550080.11824071142999977No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGCTGTAGGCACCAT1503790.11470969204255223No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCAC1490470.11369363720909356No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACC1457740.11119697994940124No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA1423790.10860726061036809No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA1345730.10265281314041441No Hit
TCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGT1322110.10085107026006204No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)