FastQCFastQC Report
Sun 2 Jul 2023
SRR1979671.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1979671.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45540350
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC14435293.1697802059053126No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT8314151.8256666889911912No Hit
TTCTGGGCTGTAGTGCGCTATGCCGAGATCGGAAGAGCACACGTCTGAAC5299971.163796501344412Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTGGGCTGTAGTGCGCTATGCCAGATCGGAAGAGCACACGTCTGAACT4150220.9113280859721105Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC3444510.7563644109015412No Hit
TTCTGGGCTGTAGTGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT2775120.6093760807723261Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT2430600.5337244882834673No Hit
TTCTGGGCTGTAGTGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC2151390.4724140240468068Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC2139680.46984267797678325Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT2096690.4604026978273114No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT2051960.45058063892789585Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC1884590.4138286157221014Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1722530.37824259146010075No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1634340.35887734723163084No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG1526780.33525873209143103No Hit
TTCTGGGCTGTAGTGCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT1357440.29807412547334394Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1321930.29027664477765325No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1263630.27747481079965347No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC1257410.2761089890613489No Hit
AGACGAATTTTTGAGCGGGTAAAGGTCGCCCTCAAGGTGACCCGCCAGAT1238100.2718687932789274No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1134660.24915487035123796No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG1080450.23725114102109449No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT967980.21255436113249018No Hit
ACTGCTCTGAAGGGGCTGAATGAAAATGGCCTTTCTGAACAGATCGGAAG932250.20470857162933528No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC906710.19910035825372444No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCACCAGATCGGAAGAGC885370.1944144039297019No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCAGATCGGAAGAGCACA883540.19401256248579554No Hit
TTCTGGGCTGTAGTGCGCTATGCCAAGATCGGAAGAGCACACGTCTGAAC834230.18318480204917179Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT830100.1822779139817766No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTCAGATCGGAAGAGCACACGT795280.1746319472731325No Hit
ATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCA786200.17263811103779395Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGCACGAGGCCCCGGGTTCAATCCCCGGCACCTCCACCAGATCGGAAGAG767550.16854284167776487No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAGATCGGAAGAGCACACGTC742150.1629653702705403No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT708800.15564219422995212No Hit
AAATGGATTTTTGGAAATAGGAGAGATCGGAAGAGCACACGTCTGAACTC691670.15188069481240263Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG684600.15032822540889562No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG680960.14952893423085242No Hit
ATTTGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAC644390.14149869291737988TruSeq Adapter, Index 19 (100% over 40bp)
TCTGGGCTGTAGTGCGCTATGCCGAGATCGGAAGAGCACACGTCTGAACT600590.13188084852224458Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACG593500.13032398740896808TruSeq Adapter, Index 19 (97% over 40bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGT569580.12507150252468416No Hit
TGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCAGATCGGAAG554300.12171623626081048No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGT553060.12144395025510343TruSeq Adapter, Index 19 (97% over 40bp)
TTCTGGGCTGTAGTGCGCTATGAGATCGGAAGAGCACACGTCTGAACTCC543140.11926566220944723Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC540420.11866838968079955No Hit
TTCTGGGCTGTAGTGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGT531110.11662404878311212Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC530930.11658452339518692No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACG511860.112397028129999No Hit
AGCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACG500600.10992449552978843No Hit
TGTAGTATCTGTTCTTATCAGTTTAATATCTGAGATCGGAAGAGCACACG475550.10442387904352952No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)