FastQCFastQC Report
Sat 1 Jul 2023
SRR1979672.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1979672.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40578913
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC17648374.34914804149633No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT15721053.8741919972080083No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG11208932.7622548686801935No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC5216781.2855888968736053No Hit
AGACGAATTTTTGAGCGGGTAAAGGTCGCCCTCAAGGTGACCCGCCAGAT3569480.8796391367112274No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG3057300.7534208715743568No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGA2957970.7289426407257384No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC2806170.6915340487311723No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC2653780.6539800610233202No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1902320.4687952089795998No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1727690.4257605421811077No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG1556870.383664786683665No Hit
ACTGCTCTGAAGGGGCTGAATGAAAATGGCCTTTCTGAACAGATCGGAAG1483020.36546567918169715No Hit
TGTGGGAAACTCGACTGCATAATTTGTGGTAGTGGGGGACTGAGATCGGA1112840.2742409585983735No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT1008220.24845909499842936No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA968210.23859929417034903No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG901660.2221991505785283No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACAC895900.22077969412339854No Hit
AATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC876900.21609745928876903Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT872280.21495893692371698Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATAGATCGG802810.19783920776783745No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAG775520.1911140399448354No Hit
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC729920.17987667634172458Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC682400.16816616058690384No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAGATCGGAAGAG662510.1632646000152838No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT652500.1607978015576711Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTGGGCTGTAGTGCGCTATGCCGAGATCGGAAGAGCACACGTCTGAAC636500.15685486696008835Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT629740.15518897709260965No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAGATCGG599270.147680151018338No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAGATCGG597330.14720207019838114No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG588080.14492256113415358No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT580160.14297080850835014No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGATCGG579060.14269973175476633No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCAGATCGGAAGAGCACA555790.13696522624940694No Hit
AGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAGATCGGAAGAGCACA555760.13695783324703645No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA554540.13665718448397077Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT550590.13568377250519253No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCG533700.13152151217061928No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGA508470.12530399717705598No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC506140.12472980732628298No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA501110.12349024726216792No Hit
TTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCAAGATCGGAAGAG493070.12150892262688259No Hit
AGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAAAAGTCGTAGATCGG462810.11405184756920422No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC461170.113647696772952No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC455540.11226027666142756No Hit
AATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAG433210.10675741856367615No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGATCGG431340.10629658808258369No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCA422290.10406636570082595No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)