FastQCFastQC Report
Wed 27 Sept 2023
SRR19898575.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19898575.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21524694
Total Bases860.9 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA217372510.098749835886169Illumina Single End Adapter 1 (95% over 22bp)
ACTGTAGAACTCTGAACCTGTAGGCACCATCAATTCGTAT1667800.7748309917901737Illumina DpnII expression PCR Primer 2 (95% over 24bp)
AAAAACCTAAAGGTAAACCTTTACTGTAGGCACCATCAAT1631070.7577668699959219No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA1387950.6448175291133058Illumina Single End Adapter 1 (95% over 22bp)
GATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCT1087900.5054194963236179No Hit
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATC935820.43476576252373206No Hit
ATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATTCG906470.42113026089941163No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA879980.4088234657366093Illumina Single End Adapter 1 (95% over 22bp)
TAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAA864820.4017803923252057Illumina Single End Adapter 1 (95% over 22bp)
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGG850610.39517867245871185No Hit
ATTTTAAAACCTAAAGGTAAACCTTTACTGTAGGCACCAT789010.3665603794413988No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGC779740.36225369800843626No Hit
TTTTGATGATTCATAATCTGTAGGCACCATCAATTCGTAT749420.3481675511856289No Hit
ATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTT748630.3478005308693355No Hit
GTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA723820.33627423460700534Illumina Single End Adapter 1 (95% over 22bp)
ATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATTCGTAT640930.2977649763569229No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCA590110.2741548846176396No Hit
AAAAACCTAAAGGTAAACCTTTATCTGTAGGCACCATCAA573750.266554311991613No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGG560550.2604218206307602No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCA554100.2574252623521617No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCAT551910.2564078262854747No Hit
TGTAGAACTCTGAACCTGTAGGCACCATCAATTCGTATGC521640.24234490859661Illumina DpnII expression PCR Primer 2 (95% over 22bp)
ATATAAAGAATGGTACCTGTAGGCACCATCAATTCGTATG489610.22746432539296493No Hit
AATACTGAATATCTAAATATTATTATTACTTCTGTAGGCA470540.21860473370724806No Hit
AGTTTTGGTGGCAGGATACTGTAGGCACCATCAATTCGTA469740.21823306756416608No Hit
AGAACTCTGAACCTGTAGGCACCATCAATTCGTATGCCGT468320.21757336016019554No Hit
TCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTC460160.21378236550075924No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCAC441500.20511325271337194No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGC439180.20403542089843413No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATC411370.19111537659954655No Hit
AATACTGAATATCTAAATATTCTGTAGGCACCATCAATTC405870.18856017186585788No Hit
TTTGATGATTCATAATCTGTAGGCACCATCAATTCGTATG368380.17114296723567823No Hit
CTGTAGAACTCTGAACCTGTAGGCACCATCAATTCGTATG354000.16446226831377952Illumina DpnII expression PCR Primer 2 (95% over 23bp)
AAAAGAATGGTACAGCCTGTAGGCACCATCAATTCGTATG352640.16383043587054014No Hit
AGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATTCG348010.16167941806745312No Hit
TAGAACTCTGAACCTGTAGGCACCATCAATTCGTATGCCG342210.15898483853010872No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG342040.15890585947470381Illumina Single End Adapter 1 (95% over 22bp)
AGTTTTGGTGGCAGGATCTGTAGGCACCATCAATTCGTAT328400.15256895173515592No Hit
ATGTACAGTAAAATTGTAAGCTGTAGGCACCATCAATTCG314500.14611125249910636No Hit
TGTACAACGGGGTATTGTAAGCCTGTAGGCACCATCAATT308220.14319367327591276No Hit
GTAGAACTCTGAACCTGTAGGCACCATCAATTCGTATGCC307010.14263152823450126Illumina DpnII expression PCR Primer 2 (95% over 21bp)
AACTTTTCGAATCGCACTGTAGGCACCATCAATTCGTATG302700.14062917688864707No Hit
GACTGTAGAACTCTGAACCTGTAGGCACCATCAATTCGTA298820.1388265960946994Illumina DpnII expression PCR Primer 2 (96% over 25bp)
AATACTGAATATCTAAATATTATTATTACTCTGTAGGCAC298720.13878013782681417No Hit
TTGATGATTCATAATCTGTAGGCACCATCAATTCGTATGC283830.13186250173870068No Hit
TTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCAC268270.12463359525575601No Hit
GATCGTCGGACTGTAGAACTCTGCACCATCAATTCGTATG267930.12447563714494618Illumina DpnII expression Sequencing Primer (96% over 27bp)
AAAAGAATGGTACAGCAATTAATATACTGTAGGCACCATC260570.1210563086285919No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACC260100.12083795476953121No Hit
ATTTTAAAACCTAAAGGTAAACCTTTATCTGTAGGCACCA258980.12031762216921645No Hit
AATACTGAATATCTAAATATTATTCTGTAGGCACCATCAA252960.11752083444252447No Hit
AGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAA248160.11529083758403255No Hit
AACTTTTCGAATCGCATCTGTAGGCACCATCAATTCGTAT234190.10880061756046333No Hit
ATCGTCGGACTGTAGAACTCTGTACCATCAATTCGTATGC233380.10842430559059282Illumina DpnII expression Sequencing Primer (96% over 26bp)
ATTTGTATACGACTTAGATCTGTAGGCACCATCAATTCGT231180.10740222369711735No Hit
ATAAAGAATGGTACAGCCTGTAGGCACCATCAATTCGTAT231050.10734182794886656No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCCTGTAGGCACCA227580.10572972605324843No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCA226990.10545562227272545No Hit
AGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAA223900.10402006179507127Illumina Single End Adapter 1 (95% over 22bp)
AAATAGATAATATACGGATCTTAATCTGTAGGCACCATCA216150.10041954603396454No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)