FastQCFastQC Report
Wed 27 Sept 2023
SRR19898576.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19898576.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25093963
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA14184305.6524750594395945Illumina Single End Adapter 1 (95% over 22bp)
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCA8236393.282219711569671No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCAC4059661.617783528253389No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCA3256301.2976427836448152No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCAT3131361.2478539160992626No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCA2700591.0761911141735563No Hit
AGTTTTGGTGGCAGGATACTGTAGGCACCATCAATTCGTA2105220.8389348466003557No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGC1969120.7846986942636361No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGC1871210.7456813417633555No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGC1844950.7352166734285852No Hit
ACTGTAGAACTCTGAACCTGTAGGCACCATCAATTCGTAT1744480.6951791552414419Illumina DpnII expression PCR Primer 2 (95% over 24bp)
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCAC1590320.633746052785684No Hit
GATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCT1452200.5787049259616746No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGG1370590.5461831596707144No Hit
ATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATTCG1254980.5001123178511102No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGG1249940.49810386665509954No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACC1184270.4719342257737449No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACC1002180.3993709562734272No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCAC997210.3973904002329166No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACC955520.3807768426214704No Hit
ACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATTC843650.33619639911001703No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGC778400.31019412916166333No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCAC745880.29723483692073666No Hit
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATC731690.2915800903986349No Hit
ATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTT723020.28812507613883065No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA673130.2682438003116527Illumina Single End Adapter 1 (95% over 22bp)
AGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAA669750.2668968628032169No Hit
AACTTTTCGAATCGCACTGTAGGCACCATCAATTCGTATG656330.26154896299161673No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCAT654520.2608276739708272No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA585120.233171619803536Illumina Single End Adapter 1 (95% over 22bp)
AGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAA579090.23076865140830882No Hit
TAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCA561170.22362749160027054No Hit
ACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATT549030.21878967463210175No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCA543840.21672144810287639No Hit
AGTTTTGGTGGCAGGATCTGTAGGCACCATCAATTCGTAT541660.21585271326015742No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATC522790.20833297634175996No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACC499900.19921126049321103No Hit
TAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAA496790.19797191858456153Illumina Single End Adapter 1 (95% over 22bp)
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGT469470.1870848378950746No Hit
AGAACTCTGAACCTGTAGGCACCATCAATTCGTATGCCGT463620.18475359989970497No Hit
CAAGACTGTAAATCTTGAGCTGTAGGCACCATCAATTCGT446280.1778435713800965No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACC443730.17682739071544817No Hit
GGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCA425350.16950291988555174No Hit
AACTTTTCGAATCGCATCTGTAGGCACCATCAATTCGTAT405730.16168430630108127No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACC393160.15667513337769726No Hit
TGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCAC387790.15453517644861436No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATC387200.15430006013797024No Hit
TGTAGAACTCTGAACCTGTAGGCACCATCAATTCGTATGC373420.14880869952665507Illumina DpnII expression PCR Primer 2 (95% over 22bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCA372490.1484380924607245No Hit
ATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATTCGTAT371980.14823485632779484No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCA367860.14659302717550032No Hit
TGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCA367390.1464057311314279No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACC364020.14506277864520642No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGC363430.1448276623345623No Hit
AGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATTCG357190.14234100847283468No Hit
TTGATGATTCATAATAACTTTTCGAATCGCCTGTAGGCAC345010.13748725141580867No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCAT337650.13455427506607864No Hit
AGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCAT318660.1269867178811095No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTA315200.12560790019495924No Hit
ACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAA311110.12397802610930764No Hit
CTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCA307420.12250755291222833No Hit
AATAACTTTTCGAATCGCACTGTAGGCACCATCAATTCGT305710.12182611411358182No Hit
TCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTC302990.1207421880712903No Hit
AAATAACTTTTCGAATCGCACTGTAGGCACCATCAATTCG291450.11614347243597992No Hit
GACTGTAGAACTCTGAACCTGTAGGCACCATCAATTCGTA289590.11540225830411881Illumina DpnII expression PCR Primer 2 (96% over 25bp)
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGG281290.11209468986624392No Hit
GATCGTCGGACTGTAGAACTCTGCACCATCAATTCGTATG275850.10992683778166087Illumina DpnII expression Sequencing Primer (96% over 27bp)
ATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATTC274330.1093211144050862No Hit
TACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCA264520.10541180761285095No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTA257200.10249477135197817No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGG251530.10023526375646605No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)