FastQCFastQC Report
Wed 27 Sept 2023
SRR19908117_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19908117_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20596835
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACAC356896117.327715641747872No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACACG9654044.687147321420985No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGATCGGAAGAGCACACG4619152.242650387790163No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACA3166331.5372895884246294No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAGATCGGAAGAGCACACG1877920.9117517327298102No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGATCGGAAGAGCACAC1711780.8310888541856066No Hit
TCACGTTCTAGCATTCAAGGTCCCGATCGGAAGAGCACACGTCTGAACTC1373960.6670733634560844Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TTTGCTCGAATATATTAGCATGGATCGGAAGAGCACACGTCTGAACTCCA1224070.5943000465848273Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAGATCGGAAGAGCA1218540.5916151680585876No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACAC1190840.5781664998530114No Hit
TCACGTTCTAGCATTCAAGGTCGATCGGAAGAGCACACGTCTGAACTCCA1172610.5693156254346845Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TAGTCACTTAGCGATGTACACTGACTGTGGATCGGAAGAGCACACGTCTG1149960.5583187902413163Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TTTTGATGATTCATAATAACTTTTCGAATCGCAGATCGGAAGAGCACACG1108690.5382817311494703No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCGATCGGAAGAGC997600.4843462599957712No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAGATCGGAAGAGCACACGT984160.47782098560288516No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGATCGGAAGAGCACAC904940.43935876555791215No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTGATCGGAAGAG904400.4390965893546266No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACAC871240.42299702842694037No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACG846210.4108446758931651No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACAC832100.40399410880360986No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACAC821660.39892536887342156No Hit
TCACGTTCTAGCATTCAAGGTCCGATCGGAAGAGCACACGTCTGAACTCC712280.3458201223634602Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGATCGGAAGAGCACACG633750.3076929052449078No Hit
TTTGCTCGAATATATTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT611350.29681744792343095Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTGCTCGAATATATTAGCATGGAATGATCGGAAGAGCACACGTCTGAAC604070.293282924293951Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TTTTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACA589510.286213877034991No Hit
TTTGCTCGAATATATTAGCATGGAATAATGATCGGAAGAGCACACGTCTG539900.2621276521368453Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TTTGCTCGAATATATTAGCGATCGGAAGAGCACACGTCTGAACTCCAGTC530450.2575395685793473Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCGATCGGAAGAGCAC528250.25647144330670224No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAGATCGGAAGAGCACACGT521370.25313112427224865No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTGATCGGAAGAGCACACGT490370.23808026815770483No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCGATCGGAAGAGCA476880.23153071819044044No Hit
AATACTGAATATCTAAATATTATTATTACTTGATCGGAAGAGCACACGTC471550.228942941961714No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGGATCGGAAG382500.18570814399396798No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACACGT374340.18174637025543003No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACG369250.17927511678371943No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGATCGGAAGAGCACACGTCT352380.1710845379884822Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGTACACGGAGTCGAGCACCCGCAACGCGAGATCGGAAGAGCACACGTC340920.16552057634097667No Hit
TCTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTGATCGGA332290.16133061220328268No Hit
AGTCACTTAGCGATGTACACTGACTGTGGATCGGAAGAGCACACGTCTGA307020.14906173691249164Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACAC275210.13361761649301945No Hit
TTTGCTCGAATATATTAGCATGGAATAGATCGGAAGAGCACACGTCTGAA269410.13080164986513704Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGCTCGAATATATTAGCATGGAATAATAGAGATCGGAAGAGCACACGTC266490.12938395632144453No Hit
TAGTCACTTAGCGATGTACACTGACTGTAGATCGGAAGAGCACACGTCTG259510.12599508613823435Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAATGATTGGAAGAGCACAC239430.11624601546791047No Hit
TTGCTCGAATATATTAGCGATCGGAAGAGCACACGTCTGAACTCCAGTCA232150.11271149183843053Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TTCACGTTCTAGCATTCAAGGTCCCGATCGGAAGAGCACACGTCTGAACT227630.11051697991463251Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TTCACGTTCTAGCATTCAAGGTCGATCGGAAGAGCACACGTCTGAACTCC223680.10859920953874709Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTGATCGGAAG223000.10826906172720227No Hit
ACGTTCTAGCATTCAAGGTCGATCGGAAGAGCACACGTCTGAACTCCAGT215360.10455975396219856Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)