FastQCFastQC Report
Wed 27 Sept 2023
SRR19908118_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19908118_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19858803
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACAC365350218.39739283379769No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACACG9007814.535927971086676No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGATCGGAAGAGCACACG3630301.8280557997377787No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACA2259511.1377876098574522No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAGATCGGAAGAGCACACG1840090.9265865621407292No Hit
TCACGTTCTAGCATTCAAGGTCCCGATCGGAAGAGCACACGTCTGAACTC1709440.8607970983951047Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TTTTGATGATTCATAATAACTTTTCGAATCGCAGATCGGAAGAGCACACG1386960.6984106746010824No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGATCGGAAGAGCACAC1332060.670765503842301No Hit
TCACGTTCTAGCATTCAAGGTCGATCGGAAGAGCACACGTCTGAACTCCA1261570.6352699102760625Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAGATCGGAAGAGCA1228050.6183907459074951No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACAC1142610.5753670047484735No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAGATCGGAAGAGCACACGT1078910.5432905497879202No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACAC935800.47122679045660504No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACG852310.42918498159229435No Hit
TCACGTTCTAGCATTCAAGGTCCGATCGGAAGAGCACACGTCTGAACTCC773020.38925810382428383Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TTTGCTCGAATATATTAGCATGGATCGGAAGAGCACACGTCTGAACTCCA765380.3854109434491092Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GTTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACAC756910.3811458324049038No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACA726300.36573201315305864No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGATCGGAAGAGCACAC684500.3446834131946422No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGATCGGAAGAGCACACG664250.33448642398033757No Hit
TTTGCTCGAATATATTAGCGATCGGAAGAGCACACGTCTGAACTCCAGTC613750.3090568953224421Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACAC566800.2854149869959433No Hit
AATACTGAATATCTAAATATTATTATTACTTGATCGGAAGAGCACACGTC551540.2777307373460525No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAGATCGGAAGAGCACACGT449470.226332876155728No Hit
TTTGCTCGAATATATTAGCATGGAATGATCGGAAGAGCACACGTCTGAAC438300.2207081665496153Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTGATCGGAAGAGCACACGT414420.2086832726020798No Hit
TTTGCTCGAATATATTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT388030.19539445554699345Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGCTCGAATATATTAGCGATCGGAAGAGCACACGTCTGAACTCCAGTCA382210.19246376531354886Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ATCCTGTCTGTGACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA370010.18632039403381964Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTGCTCGAATATATTAGCATGGAATAATGATCGGAAGAGCACACGTCTG355700.17911452165571107Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA345090.17377180286243837TruSeq Adapter, Index 27 (97% over 39bp)
TTCACGTTCTAGCATTCAAGGTCCCGATCGGAAGAGCACACGTCTGAACT341620.17202446693287607Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TTCACGTTCTAGCATTCAAGGTCGATCGGAAGAGCACACGTCTGAACTCC327340.16483370120545532Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACACGT317500.15987871977983767No Hit
ATTTTAAAACCTAAAGGTAAACCTTTAGATCGGAAGAGCACACGTCTGAA307310.154747494096195Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACG300610.15137367544257324No Hit
ATAAATCATTTGTATACGACTTAGATGATCGGAAGAGCACACGTCTGAAC292190.14713374214951425Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ATTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACAC287600.1448224245942719No Hit
TTTGCTCGAATATATTGATCGGAAGAGCACACGTCTGAACTCCAGTCACA285270.14364914139084817TruSeq Adapter, Index 1 (100% over 34bp)
ACGTTCTAGCATTCAAGGTCGATCGGAAGAGCACACGTCTGAACTCCAGT279710.14084937546336504Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TTCGGTGCCCGAGTTGTAATTTGATCGGAAGAGCACACGTCTGAACTCCA261510.13168467404606413Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TTGCTCGAATATATTAGCATGGAATAATAGAGATCGGAAGAGCACACGTC258540.1301891156279661No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATGATTGGAAGAGCACAC246530.12414141980259334No Hit
TAGTCACTTAGCGATGTACACTGACTGTGGATCGGAAGAGCACACGTCTG241510.12161357358749164Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACGTTCTAGCATTCAAGGTCCCGATCGGAAGAGCACACGTCTGAACTCCA237200.11944325143866928Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TTTGCTCGAATATATTAGCATGGAATAATAGATCGGAAGAGCACACGTCT235300.1184864968951049Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAAC224890.11324448910641795Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCGGTGCCCGAGTTGTAATTTGATCGGAAGAGCACACGTCTGAACTCC216700.10912037346863253Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TTTCGGTGCCCGAGTTGTAATTTGGATCGGAAGAGCACACGTCTGAACTC211420.1064616029475694Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TTCGGTGCCCGAGTTGTAATTTGGATCGGAAGAGCACACGTCTGAACTCC202210.10182386118639677Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)