FastQCFastQC Report
Wed 27 Sept 2023
SRR19908119_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19908119_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22270039
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACAC396069017.78483638937498No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACACG9644444.330679438864027No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGATCGGAAGAGCACACG4349041.952865911011651No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAGATCGGAAGAGCACACG2288601.027658730189022No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACA2242291.0068639754065989No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAGATCGGAAGAGCA2082460.9350949048629865No Hit
TCACGTTCTAGCATTCAAGGTCCCGATCGGAAGAGCACACGTCTGAACTC1902910.8544708879944036Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TTTTGATGATTCATAATAACTTTTCGAATCGCAGATCGGAAGAGCACACG1674210.7517768603817891No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGATCGGAAGAGCACAC1519690.6823921592593529No Hit
TCACGTTCTAGCATTCAAGGTCGATCGGAAGAGCACACGTCTGAACTCCA1382010.6205691871487068Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
CTTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACAC1346130.6044578547886692No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTA1254960.563519444218306TruSeq Adapter, Index 7 (97% over 35bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAGATCGGAAGAGCACACGT1231440.5529581694940005No Hit
TTTGCTCGAATATATTAGCATGGATCGGAAGAGCACACGTCTGAACTCCA1214880.5455221699432139Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TTTGCTCGAATATATTAGCGATCGGAAGAGCACACGTCTGAACTCCAGTC961820.43188967922328286Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GTTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACAC896430.4025273597410404No Hit
TAGTCACTTAGCGATGTACACTGACTGTGGATCGGAAGAGCACACGTCTG862060.3870940684028438Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TTTTGATGATTCATAATAACTTTTCGAATCGCATGATCGGAAGAGCACAC861000.38661809258618723No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACAC837710.37616009563342034No Hit
TCACGTTCTAGCATTCAAGGTCCGATCGGAAGAGCACACGTCTGAACTCC801920.36008917631441956Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACG759170.3408929818218998No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGATCGGAAGAGCACACG751240.33733214387276106No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACA737990.3313824461645532No Hit
TTTGCTCGAATATATTAGCATGGAATGATCGGAAGAGCACACGTCTGAAC722400.3243820093893863Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TTTGCTCGAATATATTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT642710.28859850672017234Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTGATCGGAAGAGCACACGT629780.28279249982454No Hit
TTGCTCGAATATATTAGCGATCGGAAGAGCACACGTCTGAACTCCAGTCA594440.26692364571072374Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TTGCTCGAATATATTAGCATGGAATAATAGAAGATCGGAAGAGCACACGT579830.2603632620490696No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACAC564640.25354243878962224No Hit
TTTGCTCGAATATATTAGCATGGAATAATGATCGGAAGAGCACACGTCTG550020.24697756479007513Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AATACTGAATATCTAAATATTATTATTACTTGATCGGAAGAGCACACGTC461180.20708540294877795No Hit
ATCCTGTCTGTGACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA456870.20515006731690053Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACACGT451340.2026669104620787No Hit
ATTTTAAAACCTAAAGGTAAACCTTTAGATCGGAAGAGCACACGTCTGAA425700.1911536841044598Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAAATCATTTGTATACGACTTAGATGATCGGAAGAGCACACGTCTGAAC416310.18693725682294493Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAGATCGGAAGAGCAC370500.16636701893517114No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTGATCGGAAGAG358660.16105045886987446No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGATCGGAAGAGCACACGTCT344670.15476847615758552Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCACGTTCTAGCATTCAAGGTCCCGATCGGAAGAGCACACGTCTGAACT343740.15435087473353776Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TTCACGTTCTAGCATTCAAGGTCGATCGGAAGAGCACACGTCTGAACTCC319760.14358304446615472Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCGATCGGAAGAGCA314430.14118969436919263No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCGATCGGAAGAGCAC313280.1406733055114991No Hit
AGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAAC308890.13870204717647777Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGCTCGAATATATTAGCATGGAATAATAGAGATCGGAAGAGCACACGTC304260.13662302073202476No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACAC295950.1328915499429525No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACG288580.12958217091582103No Hit
TTTGCTCGAATATATTAGCATGGAATAGATCGGAAGAGCACACGTCTGAA274420.12322385245935133Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCGATCGGAAGAGCAC268220.12043984296569933No Hit
ATTAGCATGGAATAATAGAATGATCGGAAGAGCACACGTCTGAACTCCAG261300.11733252914375228Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ACGTTCTAGCATTCAAGGTCGATCGGAAGAGCACACGTCTGAACTCCAGT257220.11550047128341355Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAATGATTGGAAGAGCACAC253930.11402315011662081No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAGATCGGAAGAGCACACGT249950.11223599563521196No Hit
ACGTTCTAGCATTCAAGGTCCCGATCGGAAGAGCACACGTCTGAACTCCA235890.10592258055767212Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TTGCTCGAATATATTAGCATGGAATGATCGGAAGAGCACACGTCTGAACT232720.10449914344559522Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TTGATGATTCATAATAACTTTTCGAATCGCAGATCGGAAGAGCACACGTC226490.10170166293826427No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTGATCGGAAGAGCACACG225330.10118078374267776No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)