FastQCFastQC Report
Wed 27 Sept 2023
SRR19908120_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19908120_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20850098
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACAC279723813.415946534160176No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACACG7827213.7540399090690126No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGATCGGAAGAGCACACG3215661.5422757245553473No Hit
TCACGTTCTAGCATTCAAGGTCCCGATCGGAAGAGCACACGTCTGAACTC2449471.1748002335528591Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACA2216821.0632180242030518No Hit
TCACGTTCTAGCATTCAAGGTCGATCGGAAGAGCACACGTCTGAACTCCA2142601.0276210692151182Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAAGATCGGAAGAGCACACG1609770.7720683135398212No Hit
TCACGTTCTAGCATTCAAGGTCCGATCGGAAGAGCACACGTCTGAACTCC1345410.6452775425803754Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TTTGCTCGAATATATTAGCATGGATCGGAAGAGCACACGTCTGAACTCCA1294830.6210186637971677Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGATCGGAAGAGCACAC1096330.5258152743454731No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAGATCGGAAGAGCACACG978880.46948460386133434No Hit
TTTGCTCGAATATATTAGCGATCGGAAGAGCACACGTCTGAACTCCAGTC947990.4546693257748717Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CTTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACAC903500.43333129657232305No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGATCGGAAGAGCACAC860550.4127318730108607No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAGATCGGAAGAGCACACGT844560.40506284430893325No Hit
TTGCTCGAATATATTAGCGATCGGAAGAGCACACGTCTGAACTCCAGTCA759910.36446351475182515Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAGATCGGAAGAGCA697760.334655501379418No Hit
TTTGCTCGAATATATTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT670650.32165316441198505Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACAC635410.3047515651964801No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACAC602440.28893868988049837No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACG540080.25902995755703406No Hit
AATACTGAATATCTAAATATTATTATTACTTGATCGGAAGAGCACACGTC537580.2578309224254006No Hit
TTCGGTGCCCGAGTTGTAATTTGATCGGAAGAGCACACGTCTGAACTCCA536440.25728416240537577Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACAC524210.251418482541425No Hit
ATCCTGTCTGTGACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA521700.250214651269265Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTGCTCGAATATATTAGCATGGAATGATCGGAAGAGCACACGTCTGAAC508760.24400844542793038Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATGTCATCTCGTA500810.24019551370933603TruSeq Adapter, Index 22 (97% over 40bp)
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGATCGGAAGAGCACACG487330.23373031627956856No Hit
TTTGCTCGAATATATTAGCATGGAATAATGATCGGAAGAGCACACGTCTG475580.22809485116089143Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TTGCTCGAATATATTAGCATGGAATAATAGAAGATCGGAAGAGCACACGT472980.22684785462399265No Hit
TTGCCCGAGTTGTAATTTGATCGGAAGAGCACACGTCTGAACTCCAGTCA466950.22395578188649282Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TTTTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACA453320.21741864234882732No Hit
ACGTTCTAGCATTCAAGGTCGATCGGAAGAGCACACGTCTGAACTCCAGT450920.21626756862245924Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TTCACGTTCTAGCATTCAAGGTCGATCGGAAGAGCACACGTCTGAACTCC411480.1973515903858102Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TTTCGGTGCCCGAGTTGTAATTTGATCGGAAGAGCACACGTCTGAACTCC410750.1970014721273732Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TTTGCTCGAATATATTGATCGGAAGAGCACACGTCTGAACTCCAGTCACC386430.18533725836684317TruSeq Adapter, Index 2 (100% over 34bp)
TTCACGTTCTAGCATTCAAGGTCCCGATCGGAAGAGCACACGTCTGAACT381740.18308786845989883Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ACGTTCTAGCATTCAAGGTCCCGATCGGAAGAGCACACGTCTGAACTCCA370540.17771619107018105Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TTCGGTGCCCGAGTTGTAATTTGGATCGGAAGAGCACACGTCTGAACTCC366410.17573538503272262Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TTTGCTCGAATATATTAGCATGGAATAATAGATCGGAAGAGCACACGTCT357100.17127017820251972Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGGTGCCCGAGTTGTAATTTGGATCGGAAGAGCACACGTCTGAACTC333000.1597114795335734Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGTGCCCGAGTTGTAATTTGATCGGAAGAGCACACGTCTGAACTCCAGTC318640.15282422173747098Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTGATCGGAAGAGCACACGT308100.1477690896225044No Hit
ATTTTAAAACCTAAAGGTAAACCTTTAGATCGGAAGAGCACACGTCTGAA298280.1430592796254483Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGCCCGAGTTGTAATTTGGATCGGAAGAGCACACGTCTGAACTCCAGT286420.13737105696097926Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACACGT284040.13622957551566425No Hit
TTGCTCGAATATATTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC278310.13348138699396042Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATAAATCATTTGTATACGACTTAGATGATCGGAAGAGCACACGTCTGAAC270660.1298123394911621Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
CCACGTTCTAGCATTCAAGGTCCCGATCGGAAGAGCACACGTCTGAACTC265820.12749100747631978Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TTTGCTCGAATATATTAGCATGGAATAGATCGGAAGAGCACACGTCTGAA262370.12583633899466562Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTGGCGTCGCTGAACCATAGGATCGGAAGAGCACACGTCTGAACTCCA255850.12270925537136565Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TTGCCCGAGTTGTAATTTGGATCGGAAGAGCACACGTCTGAACTCCAGTC249380.11960615245069832Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TTGCTCGAATATATTAGCATGGAATAATAGAGATCGGAAGAGCACACGTC237540.11392752206728236No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATGATCGGAAGAGCACAC223630.10725609059487394No Hit
AAGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAA223420.10715537164381674Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCACGTTCTAGCATTCAAGGTCCGATCGGAAGAGCACACGTCTGAACTC217600.1043640178573741Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)