FastQCFastQC Report
Fri 30 Jun 2023
SRR1991275_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1991275_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20703090
Total Bases660.3 Mbp
Sequences flagged as poor quality0
Sequence length1-48
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGA3487711.6846325838316891No Hit
TAAAGCTGAATGTGTTGGGAAACAC2480371.1980675348462475No Hit
AGCCATAGTGGGTGATAGCCCCGTAAGCG2253031.0882578397717442No Hit
GCGCCCGTAGCTCAGCTGGATAGAGCATC1672100.8076572144544606No Hit
AGCCATAGTGGGTGATAGCCCCGTAAGCGTC1490040.7197186506941717No Hit
GCGGATGTAGCTCAGTTGGTTAGAGCGCCG1321610.6383636452336342No Hit
ACGCCGGAGGTCGCGGGTTCGAGCCCCGTCAT1315930.6356200934256674No Hit
TCCGCGGTAGCTCAGTGGTAGAGCAGC828820.4003363749082866No Hit
ATAAAGCTGAATGTGTTGGGAAACAC704070.34007966926676164No Hit
GCGGTCGTGGCGGAATTGGTAGACGCGCAG695920.3361430588380768No Hit
GCCTGGGTAGCTCAGTTGGTAGAGCAGC570600.27561103197638614No Hit
GCGGGCGTAGCTCAGTGGTAGAGCGCC424890.20523023374771593No Hit
CCGGCTGGTCGTAGGTTCGAATCCTACCCGCGG405720.19597074639582787No Hit
AGGTGGGCTACACACGTGCTACAATGGCGACTACAGAGGGC401770.19406281864204813No Hit
AAAAGCTGAATGTGTTGGGAAACAC336200.16239121792930428No Hit
TCGCTGTGGGGCAACCCGTGGAAGTTCGAGTCTTCTCGAC333980.16131891422971162No Hit
CCGGCTGGTCGTAGGTTCGAATCCTACCCGC333090.16088902671050553No Hit
CCGGCTGGTCGTAGGTTCGAATCCTACCCGCG326780.15784117250130295No Hit
TGGCTGGGCACTCTAATCATACTGCCGG319650.15439724215080936No Hit
GGGTGTATAGCTCAGTTGGTAGAGCAGC310930.15018531050195888No Hit
GGGCGCCAGTTCGAGTGGAGCCATCCTTGAAATACCACCCTT306420.14800689172485845No Hit
GCGGATGTAGCTCAGTTGGTTAGAGCGCC288740.1394671037028772No Hit
TAGCCATAGTGGGTGATAGCCCCGTAAGCG287450.13884400830987065No Hit
CCGAGGGGGTCACAGGTTCAATCCCTGTCGCATC268830.12985018178445829No Hit
AGGAGTGTAGCTCAGCTGGTAGAGCATC267180.12905319930503126No Hit
GCGGTCGTGGCGGAATTGGTAGACGCGCA260960.1260488168674338No Hit
CCGGAGGTCGCGGGTTCGAGCCCCGTCAT257680.12446451230226985No Hit
AGGTGGGCTACACACGTGCTACAATGGCGAC253380.1223875276589147No Hit
GGTCCCTTCGTCTATCGGTTAGGACGCC241920.1168521220745309No Hit
CGGGCGCCAGTTCGAGTGGAGCCATCCTTGAAATACCACCCTT241890.11683763148399587No Hit
AAAGCTGAATGTGTTGGGAAACAC239160.1155189877453076No Hit
CGGAGCGTGGCGCAGCCTGGTAGCGCACTTGA237510.1147220052658806No Hit
CGGAGGTCGCGGGTTCGAGCCCCGTCAT226550.10942810952374743No Hit
AGGTGGGCTACACACGTGCTACAATGGCGACTACAGAGGG216170.10441436519862494No Hit
ACGCCGGAGGTCGCGGGTTCGAGCCCCGTCATTCG210280.10156937925691287No Hit
CGCCGGAGGTCGCGGGTTCGAGCCCCGTCAT207950.10044394339202506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)