FastQCFastQC Report
Fri 30 Jun 2023
SRR1991277_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1991277_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22993484
Total Bases674.6 Mbp
Sequences flagged as poor quality0
Sequence length1-48
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAGCTGAATGTGTTGGGAAACAC13392055.824280478765202No Hit
ATAAAGCTGAATGTGTTGGGAAACAC4393081.910576057112528No Hit
AAAAGCTGAATGTGTTGGGAAACAC2868941.24771870152431No Hit
GCGGATGTAGCTCAGTTGGTTAGAGCGCCG1617570.7034906062952443No Hit
ACGCCGGAGGTCGCGGGTTCGAGCCCCGTCAT1580480.6873599494535061No Hit
GCGCCCGTAGCTCAGCTGGATAGAGCATC1259180.5476247096786202No Hit
ATGTACACGGAGTCGACCCGCAACGCGA923330.40156159023138904No Hit
TGGCTGGGCACTCTAATCATACTGCCGG914190.39758655104202567No Hit
TTAAAGCTGAATGTGTTGGGAAACAC899180.3910586146927538No Hit
AGCCATAGTGGGTGATAGCCCCGTAAGCG846490.36814342706829467No Hit
AGCCATAGTGGGTGATAGCCCCGTAAGCGTC821140.3571185645463732No Hit
AGGTGGGCTACACACGTGCTACAATGGCGAC800200.34801163668802865No Hit
AAAGCTGAATGTGTTGGGAAACAC758650.32994129989174326No Hit
AACCCGTGGAAGTTCGAGTCTTCTCGAC733160.31885555055510506No Hit
AGCCATCCTTGAAATACCACCCTTATTG699840.3043644886525243No Hit
ACGCCGGAGGTCGCGGGTTCGAGCCCCGTCATTCG512610.22293707208529165No Hit
AGTGGGTGATAGCCCCGTAAGCGTC500630.2177269003688175No Hit
AGGTCGTGGAGGTTCGAGTCCTCTCCTGGGCACCA484610.21075970914194644No Hit
TTGGCTGGGCACTCTAATCATACTGCCGG478910.20828074597133692No Hit
ATAGTGGGTGATAGCCCCGTAAGCGTC472560.20551909401811402No Hit
ATAGTGGGTGATAGCCCCGTAAGCG463700.20166582845818404No Hit
TCCGCGGTAGCTCAGTGGTAGAGCAGC459680.19991750706417521No Hit
AGTGGGTGATAGCCCCGTAAGCG427230.18580481322447698No Hit
ACTGGACCCGGGTGCGATTCCCGGCGGCTC416010.1809251699307508No Hit
ACCGGCTGGTCGTAGGTTCGAATCCTACCCGCGG403690.17556713023567894No Hit
AGGCGCGGGAGAGTAGGTCGCCGCCAGGTCC350390.1523866500613826No Hit
ACGCATGGAGCTTACCGGTACTAATAGCTCG329130.1431405523408284No Hit
ACGATGAGTGCTAGTTGTCGGCATG329040.14310141081708191No Hit
AAGCTGAATGTGTTGGGAAACAC327710.14252298607727304No Hit
ATGGAGCTTACCGGTACTAATAGCTCG302750.1316677368249196No Hit
AGGAGTGTAGCTCAGCTGGTAGAGCATC290390.12629230089707155No Hit
AACCCGTGGAAGTTCGAGTCTTCTCGACCGCACCA277890.12085597815450673No Hit
ACCGAGGGGGTCACAGGTTCAATCCCTGTCGCATC274820.11952081728893282No Hit
AGGCTGAGGTCGGCAGTTCAATCCTGCCCGGCGG268490.116767863452098No Hit
GCCTGGGTAGCTCAGTTGGTAGAGCAGC267130.11617639153770694No Hit
GCGGTCGTGGCGGAATTGGTAGACGCGCAG258510.11242750337443425No Hit
AGGCCTGTAGCTCAGGTGGTTAGAGCGTA255360.11105755004330792No Hit
AGCTCAGTTGGTTAGAGCGCCG254250.11057480458376817No Hit
AGTCGATGGGAATCAGGTGAATATTCCTG247390.10759135066264859No Hit
CCGGAGGTCGCGGGTTCGAGCCCCGTCAT247270.10753916196431998No Hit
AGCTCAGCTGGATAGAGCATC244560.10636056719373194No Hit
AACGAGCGCAACCCTCGTGATTAGTTGCCATC232960.10131565968863178No Hit
ACCAGTTGGAAGTGACGGATCTGGTAAATTGTCAG231960.1008807538692266No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)