FastQCFastQC Report
Fri 30 Jun 2023
SRR1991278_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1991278_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17278060
Total Bases518 Mbp
Sequences flagged as poor quality0
Sequence length1-48
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCATAGTGGGTGATAGCCCCGTAAGCG212511112.299476908865927No Hit
TAAAGCTGAATGTGTTGGGAAACAC12240527.084429617676985No Hit
AGCCATAGTGGGTGATAGCCCCGTAAGCGTC8124894.702431870244691No Hit
AAAAGCTGAATGTGTTGGGAAACAC1732141.0025083834643473No Hit
GCGCCCGTAGCTCAGCTGGATAGAGCATC1655740.9582904562202007No Hit
AAAGCTGAATGTGTTGGGAAACAC1501030.8687491535508037No Hit
TAGCCATAGTGGGTGATAGCCCCGTAAGCG1298850.7517337015845529No Hit
ATAAAGCTGAATGTGTTGGGAAACAC1239620.7174532325967151No Hit
TCCGCGGTAGCTCAGTGGTAGAGCAGC813500.47082832216116854No Hit
ATAGTGGGTGATAGCCCCGTAAGCG757990.43870087266741753No Hit
AGCCATAGTGGGTGATAGCCCCGTAAGC754070.4364320994370896No Hit
AGCCATAGTGGGTGATAGCCCCGTAAGCGT749320.43368294820136055No Hit
AACACAGCCATAGTGGGTGATAGCCCCGTAAGCG666790.38591716894142053No Hit
GCGGATGTAGCTCAGTTGGTTAGAGCGCCG614470.35563599154071696No Hit
AGGTGGGCTACACACGTGCTACAATGGCGAC534010.30906826345087357No Hit
AGTGGGTGATAGCCCCGTAAGCG491580.28451110830729837No Hit
GCGGTCGTGGCGGAATTGGTAGACGCGCAG446500.25842021615852706No Hit
TAGCCATAGTGGGTGATAGCCCCGTAAGCGTC445570.25788196128500535No Hit
AGCCATAGTGGGTGATAGCCCCGTAAGCGTCAAATAGCG407160.23565145623987876No Hit
ATAGTGGGTGATAGCCCCGTAAGCGTC404190.23393251325669662No Hit
GCGGGCGTAGCTCAGTGGTAGAGCGCC393920.22798855890070993No Hit
TTAAAGCTGAATGTGTTGGGAAACAC383600.22201566611066287No Hit
GCCTGGGTAGCTCAGTTGGTAGAGCAGC350990.2031420194165317No Hit
AGGAGTGTAGCTCAGCTGGTAGAGCATC319820.18510179962333734No Hit
AGTGGGTGATAGCCCCGTAAGCGTC303740.17579519922954315No Hit
ACCAGTTGGAAGTGACGGATCTGGTAAATTGTCAGG293180.16968340195600665No Hit
ACCCATAGTGGGTGATAGCCCCGTAAGCG289770.1677098007530938No Hit
AACCCGTGGAAGTTCGAGTCTTCTCGAC281970.16319540503968616No Hit
GCGGTCGTGGCGGAATTGGTAGACGCGCA277300.16049255529845366No Hit
AGCCATAGTGGGTGATAGCCCCGTAAGCGTCA276280.15990221124362342No Hit
ACGATGAGTGCTAGTTGTCGGCATG272170.15752347196386632No Hit
AGCCATCCTTGAAATACCACCCTTATTG258310.14950173804234967No Hit
CGGAGCGTGGCGCAGCCTGGTAGCGCACTTGA250490.14497576695531791No Hit
TGGCTGGGCACTCTAATCATACTGCCGG240940.13944852604979957No Hit
GCCATCCTTGAAATACCACCCTTATTG233260.1350035825781367No Hit
AGGCCTGTAGCTCAGGTGGTTAGAGCGTA229050.13256696643025895No Hit
ACGCCGGAGGTCGCGGGTTCGAGCCCCGTCAT227610.13173353952932215No Hit
AACCAGTTGGAAGTGACGGATCTGGTAAATTGTCAGG221380.1281278106454081No Hit
AGGCGCGGGAGAGTAGGTCGCCGCCAGGTCC221140.12798890616191866No Hit
ACCAGTTGGAAGTGACGGATCTGGTAAATTGTCAG216480.1252918441074982No Hit
AGGTCGTGGAGGTTCGAGTCCTCTCCTGGGCACCA215480.12471307542629208No Hit
ACGCCGGAGGTCGCGGGTTCGAGCCCCGTCATTCG186470.10792299598450289No Hit
AACCAGTTGGAAGTGACGGATCTGGTAAATTGTCAG180560.1045024730785748No Hit
AGGTGGGCTACACACGTGCTACAATGGCGACTACAGAGGGC177460.10270829016683586No Hit
AGCCATAGTGGGTGATAGCCCCGTAAG175740.10171280803516136No Hit
AACACAGCCATAGTGGGTGATAGCCCCGTAAGCGTC175350.10148708824949099No Hit
TAGTGGGTGATAGCCCCGTAAGCG172890.10006331729372395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)