FastQCFastQC Report
Fri 30 Jun 2023
SRR1991279_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1991279_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20443469
Total Bases604.2 Mbp
Sequences flagged as poor quality0
Sequence length1-48
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAGCTGAATGTGTTGGGAAACAC16626048.132690200474293No Hit
ATAAAGCTGAATGTGTTGGGAAACAC5408912.645788735757126No Hit
AGCCATAGTGGGTGATAGCCCCGTAAGCGTC5404112.643440797645449No Hit
AGCCATAGTGGGTGATAGCCCCGTAAGCG3281491.6051532154352084No Hit
AAAGCTGAATGTGTTGGGAAACAC2342911.1460432669230451No Hit
AAAAGCTGAATGTGTTGGGAAACAC2331421.1404228900682167No Hit
GCGCCCGTAGCTCAGCTGGATAGAGCATC2041240.9984802481418394No Hit
TCCGCGGTAGCTCAGTGGTAGAGCAGC1751450.8567283761870356No Hit
GCGGGCGTAGCTCAGTGGTAGAGCGCC1005680.49193216669832307No Hit
ACGCCGGAGGTCGCGGGTTCGAGCCCCGTCAT890600.4356403504708521No Hit
ACGATGAGTGCTAGTTGTCGGCATG803310.392942117602448No Hit
ATAGTGGGTGATAGCCCCGTAAGCGTC799150.3909072379056608No Hit
AGTGGGTGATAGCCCCGTAAGCGTC797590.39014415801936553No Hit
GCGGATGTAGCTCAGTTGGTTAGAGCGCCG712480.3485122803766817No Hit
GCGGTCGTGGCGGAATTGGTAGACGCGCAG553630.27081020349335033No Hit
GCCTGGGTAGCTCAGTTGGTAGAGCAGC543280.2657474619400455No Hit
GAATGTGTTGGGAAACAC399060.19520170475959828No Hit
ATAGTGGGTGATAGCCCCGTAAGCG390680.19110259613962777No Hit
AGGAGTGTAGCTCAGCTGGTAGAGCATC386620.1891166318201671No Hit
AGTGGGTGATAGCCCCGTAAGCG359490.1758458899514559No Hit
GCGGTCGTGGCGGAATTGGTAGACGCGCA356510.1743882117071227No Hit
AGCCATAGTGGGTGATAGCCCCGTAAGCGTCA313440.1533203586925487No Hit
ACCGAGGGGGTCACAGGTTCAATCCCTGTCGCATC307980.1506495790905154No Hit
AGCCATAGTGGGTGATAGCCCCGTAAGCGT306270.14981312613823025No Hit
TTAAAGCTGAATGTGTTGGGAAACAC290810.14225080880353524No Hit
TGAATGTGTTGGGAAACAC287570.14066595057815287No Hit
AACCCGTGGAAGTTCGAGTCTTCTCGAC286800.1402893021727379No Hit
CGGAGCGTGGCGCAGCCTGGTAGCGCACTTGA286070.1399322199182536No Hit
AACACAGCCATAGTGGGTGATAGCCCCGTAAGCGTC264610.12943497994396155No Hit
CGGAGGTCGCGGGTTCGAGCCCCGTCAT264460.12936160687797163No Hit
TAAAGCTGAATGTGTTGGGAAACA255350.12490541600351682No Hit
AACGATGAGTGCTAGTTGTCGGCATG240440.1176121332441182No Hit
ATCTAAATGCGAACGATAACTTCGCTG234020.11447176601974939No Hit
AGCCATAGTGGGTGATAGCCCCGTAAGC230450.11272548704918915No Hit
AGCCATAGTGGGTGATAGCCCCGTAAGCGTCAAATAGC229570.11229503172871493No Hit
CCGGAGGTCGCGGGTTCGAGCCCCGTCAT221380.10828886232566498No Hit
AACACAGCCATAGTGGGTGATAGCCCCGTAAGCG207920.10170485253750232No Hit
AGGTGGGCTACACACGTGCTACAATGGCGAC204770.10016401815171389No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)