FastQCFastQC Report
Fri 30 Jun 2023
SRR1991280_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1991280_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13538820
Total Bases382.4 Mbp
Sequences flagged as poor quality0
Sequence length1-48
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAGCTGAATGTGTTGGGAAACAC11073718.179228322704638No Hit
AGCCATAGTGGGTGATAGCCCCGTAAGCGTC6452384.76583631365215No Hit
ATGTACACGGAGTCGACCCGCAACGCGA3481852.5717529297235653No Hit
ATAAAGCTGAATGTGTTGGGAAACAC3455202.552068791814944No Hit
AGCCATAGTGGGTGATAGCCCCGTAAGCG1693081.250537343727149No Hit
GCGCCCGTAGCTCAGCTGGATAGAGCATC1487521.0987072728642526No Hit
AAAGCTGAATGTGTTGGGAAACAC1295780.9570848862751702No Hit
TCCGCGGTAGCTCAGTGGTAGAGCAGC1156140.8539444353348371No Hit
AAAAGCTGAATGTGTTGGGAAACAC1088080.8036741754451274No Hit
ACGATGAGTGCTAGTTGTCGGCATG768830.5678707597855648No Hit
GAATGTGTTGGGAAACAC668520.4937801078675985No Hit
AGCCATAGTGGGTGATAGCCCCGTAAGCGTCA555110.4100135757769141No Hit
GCGGGCGTAGCTCAGTGGTAGAGCGCC537780.3972133465102572No Hit
GGATGCGTAGCTCAGCGGGAGAGCACC370480.27364275468615434No Hit
ACGCCGGAGGTCGCGGGTTCGAGCCCCGTCAT359460.26550319747215784No Hit
ATAGTGGGTGATAGCCCCGTAAGCGTC357510.26406289469835625No Hit
AGTGGGTGATAGCCCCGTAAGCGTC341720.25240013531459904No Hit
AGCCATAGTGGGTGATAGCCCCGTAAGCGT340030.25115187291063773No Hit
AGCCATAGTGGGTGAT332610.245671336202121No Hit
GCGGTCGTGGCGGAATTGGTAGACGCGCAG328880.2429162955117211No Hit
AGCCATAGTGGGTGATAGCCCCGTAAGCGTCAAATAGC321960.23780506720674327No Hit
CGGAGCGTGGCGCAGCCTGGTAGCGCACTTGA310970.22968766849695912No Hit
GCCTGGGTAGCTCAGTTGGTAGAGCAGC308910.22816611787437902No Hit
CGGAGGTCGCGGGTTCGAGCCCCGTCAT288460.21306140416963962No Hit
GCGGATGTAGCTCAGTTGGTTAGAGCGCCG287900.21264777875767607No Hit
TGAATGTGTTGGGAAACAC266800.19706296412833613No Hit
GGAGGTCGCGGGTTCGAGCCCCGTCAT224520.16583424552509007No Hit
GCGGTCGTGGCGGAATTGGTAGACGCGCA221150.16334510688523815No Hit
GGGTGTATAGCTCAGTTGGTAGAGCAGC218970.1617349222458087No Hit
GCCCAGGTGGCGGAATTGGTAGACGCGCT209330.1546146562255795No Hit
AACGATGAGTGCTAGTTGTCGGCATG204650.15115792956845575No Hit
GGTCCCTTCGTCTATCGGTTAGGACGCC201090.14852845373525905No Hit
AGCCCCGTAAGCGTC197400.14580295771714227No Hit
CCGAGGGGGTCACAGGTTCAATCCCTGTCGCATC190610.1407877495970845No Hit
TTAAAGCTGAATGTGTTGGGAAACAC183070.1352185788717185No Hit
GGAGACGTGGCCGAGAGGCTGAAGGCACC182560.13484188430010888No Hit
AGCTCAGCTGGATAGAGCATC181230.1338595239466955No Hit
GGGGCCGATCAGCATCGACAGACGTG180340.13320215498839633No Hit
TGGCTGGGCACTCTAATCATACTGCCGG177630.13120050344121573No Hit
ACCGAGGGGGTCACAGGTTCAATCCCTGTCGCATC176490.1303584802811471No Hit
GGATGCGTAGCTCAGCGGGAGAGCACCT175520.1296420219782817No Hit
AGGAGTGTAGCTCAGCTGGTAGAGCATC174250.128703978633293No Hit
AACACAGCCATAGTGGGTGATAGCCCCGTAAGCGTC160470.118525839031762No Hit
ATCTAAATGCGAACGATAACTTCGCTG156770.11579295684557443No Hit
TGGACCGCCACAGAGATGTGG153880.11365835427311981No Hit
GCCATCCTTGAAATACCACCCTTATTG143400.10591765013494528No Hit
CCTGCGGCTGGATCACCTCC141250.10432962399972819No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)