FastQCFastQC Report
Sat 23 Sept 2023
SRR19918519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19918519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19309568
Total Bases965.4 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1523090.7887747669963409No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA1085780.5623015491594633No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1075380.5569156182054409No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1061560.5497585445723074No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1015960.5261433088508246No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA964570.49952955964628515No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA797310.41290928932226756No Hit
CGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA747450.38708789342154104No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA741180.3838407985098372No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA733130.37967188080023334No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA669290.3466105507901575No Hit
GGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA578660.29967526979371056No Hit
CCGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG559570.2897889792252214No Hit
CCGTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAA552920.2863450906825052No Hit
CCGCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA531870.27544375928037335No Hit
CCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA523590.2711557296362094No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA516780.2676289806172774No Hit
CGCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA500580.25923935740043486No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAAA491360.2544645224585035No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA488480.25297303388662035No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA476530.24678439206925812No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA461610.23905765266214138No Hit
GGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA461240.23886603781089252No Hit
CGGCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA454030.23513213760142124No Hit
CGCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG449990.23303991057697404No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA422150.21862218771543726No Hit
GCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA410550.21261480318979692No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA410020.2123403278623323No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA406340.21043453690937053No Hit
CGGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG397050.20562345050909475No Hit
GGGCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA395660.20490360012197065No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAAA387660.20076057631118419No Hit
CCGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA384400.1990722941082887No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA377130.1953073212202365No Hit
CCCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG375570.19449943157713317No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA374550.1939711960412579No Hit
GGCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA371940.19261953452298883No Hit
GCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA368230.1906982072307366No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA349450.18097245883491542No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA343650.17796876657209523No Hit
CGCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAAA339400.17576778517261496No Hit
GCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA333200.17255694171925545No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA329030.17039739055788303No Hit
CGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA321410.16645116037810892No Hit
GGGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG314640.1629451264782309No Hit
GGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAAG305650.15828940347085962No Hit
GCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAA302270.15653897591080235No Hit
GGCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG296330.1534627807312934No Hit
CGCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAA289390.1498687075754362No Hit
GCGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG287800.14904528159304237No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA285790.14800434686058228No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA285260.14772987153311767No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA280150.14508351507397782No Hit
CCGGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA277620.14377328379381663No Hit
GCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA276920.1434107692103728No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA270530.14010152894150713No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG270420.1400445623641088No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAAA268780.1391952424828976No Hit
GGGGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA268070.1388275491196903No Hit
GGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAAA264400.13692693694649202No Hit
GGGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAA259150.13420807757066341No Hit
CCGTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCG256340.13275284045712468No Hit
GCGCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA245900.12734619438404837No Hit
CCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAAA243170.1259323875086175No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA240810.12471019548443549No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA239020.12378319390677203No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAA235910.12217259340032878No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGAT235900.12216741462056531No Hit
CCGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAA230750.11950034304237153No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA229180.1186872746195047No Hit
CGGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA224850.11644486298191653No Hit
GCGTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAA222190.11506730756483004No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG220330.11410405452882219No Hit
CGCTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCG220310.11409369696929522No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGAT219600.11372600360608792No Hit
GCCGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTT218210.11300615321896378No Hit
CGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAAAA211900.10973834318820598No Hit
GGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAAA210760.10914796229516889No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAAAA210210.10886312940817733No Hit
CGGTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAA209020.10824685461632284No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG208430.10794130661027734No Hit
GCCTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCG207390.10740271351487511No Hit
CCGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAAG205420.10638249390146895No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAAA204900.10611319735376783No Hit
GCCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG202150.10468903291880999No Hit
CCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAAA199950.10354970137084372No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA197050.10204785523943362No Hit
GGCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAA195190.10108460220342579No Hit
CCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA194010.10047350619133479No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA193360.10013688550670838No Hit

[FAIL]Adapter Content

Adapter graph