FastQCFastQC Report
Sat 23 Sept 2023
SRR19918528_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19918528_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13079260
Total Bases653.9 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA2378381.8184362112229588No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA2151051.6446266837726293No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1918431.4667725849933406No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1452821.110781496812511No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1318591.0081533664748616No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA985220.753268915825513No Hit
GCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA784540.5998351588698443No Hit
CGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA704100.5383332084536893No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA664190.5078192497129043No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA626300.47884972085576705No Hit
CGCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA589140.4504383275506413No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA569420.4353610219538414No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA519880.3974842613420025No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA496430.3795551124451995No Hit
GCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA443320.3389488396132503No Hit
GGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA437080.3341779274974272No Hit
CGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA413010.3157747456660392No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA408810.3125635548188506No Hit
CGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA408270.31215068742421204No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA387600.29634704104054815No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA378930.2897182256488517No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA373930.28589537940219856No Hit
CCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA362260.2769728562625103No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA342820.262109630055523No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG335130.2562300925281705No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA334500.25574841390109226No Hit
CCGTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCG311830.23841562901876714No Hit
GCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA311730.23833917209383407No Hit
GGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA307450.235066815706699No Hit
GGCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA306470.234317537842355No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG304220.23259725703136108No Hit
CGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA302710.2314427574648719No Hit
GGGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAA298690.22836918908256276No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA291410.22280312494743587No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA290420.22204620139059855No Hit
CGCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG286140.21877384500346347No Hit
CGCTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCG283250.21656423987289802No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGAT276450.21136516897744978No Hit
GGGGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA262260.2005159313294483No Hit
CCGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA261540.19996544146993028No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA258870.19792404157421753No Hit
GGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA257860.19715182663239358No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA250630.1916239909597332No Hit
CCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA243340.18605028113211297No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA232990.17813698940154107No Hit
CCCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG229640.1755756824162835No Hit
CCGGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA225950.1727544218862535No Hit
CCGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAA225520.17242565710904134No Hit
CCCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA223680.171018849690273No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA219690.16796821838544382No Hit
CCGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG214750.16419124629375056No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA213840.1634954882768597No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA209790.16039898281707068No Hit
GCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA206870.15816644060902527No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGAT206190.15764653351948046No Hit
GCCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA201810.15429772020741236No Hit
GGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA199330.15240158846907242No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA193300.14779123589560877No Hit
GCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA184390.14097892388407296No Hit
CGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAGATCGGAAGA183560.14034433140712854No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG178680.1366132334703951No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA171410.1310548150277615No Hit
GGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAAG170300.1302061431610045No Hit
GCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA158720.12135243125375596No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA158130.12090133539665088No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG157770.12062609046689185No Hit
CCGCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA156570.11970860736769512No Hit
CGCCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA151960.11618394312828097No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAGATC151180.11558757911380307No Hit
CGGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA150070.11473890724704608No Hit
CCGCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAGATCGGAAGA149320.11416548031004813No Hit
CGCCCCCTTGTAAATTTAACTGTTAGTCCAAAAAAAAAAAAAAAGATCGG144900.11078608422800677No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA143160.1094557337341715No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA142690.10909638618698612No Hit
GGCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG141070.10785778400307051No Hit
CGGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG140340.10729964845105915No Hit
CGGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAA140240.1072231915261261No Hit
CGGCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA136840.104623656078402No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)