FastQCFastQC Report
Sat 23 Sept 2023
SRR19918529_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19918529_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13046003
Total Bases652.3 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA2309341.770151363601557No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA2102661.6117273620127177No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1860911.4264215637540478No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1402731.075218210512446No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1272540.9754251934481388No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA949140.7275331762532938No Hit
GCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA757550.5806759357636204No Hit
CGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA675150.5175148281048226No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA644980.49438897108945934No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA605900.4644334360493402No Hit
CGCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA570000.43691542919314064No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA552380.42340937680299473No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA503640.3860492750154971No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA482760.3700443729776852No Hit
GGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA425570.3262071915819734No Hit
GCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA425460.32612287456932215No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA394170.3021385170615092No Hit
CGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA393220.3014103246795206No Hit
CGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA389910.29887314911701307No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA377980.28972858583583033No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA364110.2790969770587972No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA360710.276490814849575No Hit
CCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA341300.26161269470810333No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA333040.2552812535762869No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA328480.2517859301427418No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG315790.2420588129559682No Hit
CCGTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCG303370.2325386557093387No Hit
GGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA300050.22999381496386287No Hit
GCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA299270.22939593069233544No Hit
GGCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA299160.2293116136796841No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG289040.22155444851576378No Hit
CGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA287770.2205809702787896No Hit
GGGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAA281850.21604318196155559No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA281580.21583622202141148No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA275570.21122944705746272No Hit
CGCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG273370.20954310680443658No Hit
CGCTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCG268800.20604011818792317No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGAT261470.2004215390721587No Hit
CCGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA253270.19413608903815213No Hit
GGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA250030.19165256975642272No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA249350.19113133731457826No Hit
GGGGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA248300.1903264931029067No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA247750.18990490803965016No Hit
CCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA238330.18268430568351088No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA226750.17380802380621865No Hit
CCCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG222630.17064996842327876No Hit
CCGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAA214840.1646787908909725No Hit
CCGGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA214820.16466346052503592No Hit
CCCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA213390.16356733936056891No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA213090.1633373838715199No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA206910.15860030079711004No Hit
CCGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG205200.15728955450953064No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA202340.1550973121805966No Hit
GCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA199660.15304304314509204No Hit
GCCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA192960.14790737055633055No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGAT192710.14771574098212303No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA190570.14607539182690668No Hit
GGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA190380.14592975335050898No Hit
GCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA181630.13922271825324584No Hit
CGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAGATCGGAAGA175170.13427101005572356No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG170280.13052273558422453No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA163450.12528741561687515No Hit
GGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAAG162600.12463587506456959No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG155090.11887932265537576No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA154600.11850372868992902No Hit
GCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA153180.1174152727084303No Hit
CCGCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA150970.11572126727243585No Hit
CGCCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA145480.11151308182283876No Hit
CGGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA144070.11043229102430836No Hit
CCGCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAGATCGGAAGA144000.11037863474353025No Hit
CGCCCCCTTGTAAATTTAACTGTTAGTCCAAAAAAAAAAAAAAAGATCGG142390.10914454028563385No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAGATC142070.10889925443064823No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA141360.10835502643989887No Hit
GGCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG138240.10596348935378905No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA136630.10472939489589263No Hit
CGGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG135320.10372525592704525No Hit
CGGCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA131280.10062852200785175No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)