FastQCFastQC Report
Sat 23 Sept 2023
SRR19918531_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19918531_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13176344
Total Bases658.8 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA2366651.7961355593023376No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA2135731.620882089902935No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1908141.4481558769261034No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1446331.0976717062031773No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1311960.9956934943410706No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA984600.7472482503492622No Hit
GCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA778240.5906342457361465No Hit
CGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA698320.5299800915944514No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA661210.5018159817321102No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA625580.47477509694646713No Hit
CGCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA590000.44777215895395567No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA566250.4297474322164024No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA517320.3926127004577294No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA501030.38024963525542443No Hit
GCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA443200.33636037431931043No Hit
GGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA439020.33318802241350104No Hit
CGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA411990.3126739860465088No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA406360.3084011771398804No Hit
CGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA402830.305722133544783No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA389880.29589391412367494No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA377410.28642998391663116No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA368250.27947813141490535No Hit
CCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA357050.27097804975340656No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA338410.2568314852739121No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA336640.2554881687970502No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG331250.2513975044974539No Hit
CCGTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCG315390.2393607817160815No Hit
GCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA314990.23905720737102798No Hit
GGCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA310230.23544467266489094No Hit
GGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA308710.23429109015368757No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG300840.22831826491475937No Hit
CGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA299460.22727093342432467No Hit
GGGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAA292880.22227713544819414No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA291660.22135123369578089No Hit
CGCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG283060.21482438527713No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA282570.21445250670443938No Hit
CGCTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCG279340.21200114386813215No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGAT270370.20519348918030678No Hit
GGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA264600.2008144292529096No Hit
CCGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA261040.1981126175819332No Hit
GGGGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA257910.19573714833188935No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA257100.19512241028315594No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA255950.19424963404112702No Hit
CCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA243770.18500579523424707No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA232650.17656642844175896No Hit
CCCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG226000.17151950495524404No Hit
CCGGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA222350.1687493890566306No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA222070.16853688701509312No Hit
CCGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAA221850.16836992112531365No Hit
CCCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA218360.16572123496472163No Hit
CCGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG213280.1618658407825418No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA210340.15963456934639836No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA209890.15929304820821313No Hit
GCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA205640.15606757079201938No Hit
GCCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA201850.1531912038726372No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGAT201640.1530318273414841No Hit
GGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA197420.14982911800116935No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA194290.1474536487511255No Hit
GCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA190410.14450897760410628No Hit
CGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAGATCGGAAGA184480.1400084879386877No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG174560.13247984418136016No Hit
GGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAAG172470.13089366822845547No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA171550.13019544723483237No Hit
CCGCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA160700.12196099312525538No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG160070.12148286353179608No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA157580.11959311323383784No Hit
GCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA157240.11933507504054235No Hit
CGCCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA151640.11508503420979295No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAGATC149910.11377207516743643No Hit
CGGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA148680.11283858405639682No Hit
CCGCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAGATCGGAAGA148520.11271715431837542No Hit
CGCCCCCTTGTAAATTTAACTGTTAGTCCAAAAAAAAAAAAAAAGATCGG143140.10863407937740543No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA142790.1083684518254836No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA142010.10777648185262922No Hit
CGGCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA139460.10584119540291298No Hit
GGCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG139200.10564387207862819No Hit
CGGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG138760.10530994029906929No Hit
CGGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAA134310.10193267571034878No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)