FastQCFastQC Report
Sat 23 Sept 2023
SRR19918539_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19918539_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25663871
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1758870.6853486755758708No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1340700.5224075510666337No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1300890.5068954718483428No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1292710.503708111687438No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA1202570.4685848054644602No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1170940.45626008640707394No Hit
CGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA1078530.4202522682568035No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA1062920.4141697875585488No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA962560.37506422939859696No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA913650.3560063094145073No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA911380.3551217974872146No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA831070.32382877859696224No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA812200.31647602966832244No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA789810.3077517027731319No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA716670.2792524946840638No Hit
CCGTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAA715650.2788550487960293No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA698820.2722971916434586No Hit
CGCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA688410.26824090566851744No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA679120.26462103086475147No Hit
GGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA673900.2625870430848098No Hit
CCGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG639080.249019331495237No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAAA593840.23139143740240903No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA575160.22411272251173642No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA567650.22118642974787395No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA565750.2204460893682017No Hit
CCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA561730.2188796849859478No Hit
GGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA560180.21827572309726775No Hit
CCGCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA559520.2180185522285395No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA550880.2146519517651877No Hit
CGCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG538730.2099176698635993No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA527070.20537431784940002No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAA506260.1972656424278317No Hit
CGGCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA505330.19690326529462374No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA503600.19622916589629055No Hit
CCCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG496630.19351328566138756No Hit
GCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA492890.19205598407192742No Hit
GGCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA490580.1911558860313785No Hit
CCGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA480750.18732559869865306No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA473630.1845512705390391No Hit
CGGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG463680.18067422486654489No Hit
GCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA457050.1780908265943201No Hit
GGGCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA439000.17105759298743356No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAA431890.16828716135613367No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA429010.1671649612016831No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAA423560.16504135327051792No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA421960.1644179087402676No Hit
GGGGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA421780.16434777123061445No Hit
GCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA415600.16193971673252253No Hit
GGGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAA411610.16038500193521077No Hit
CCGGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA405820.15812891204136742No Hit
GCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA397690.15496103452203294No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA395120.15395962674531835No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAAA390640.15221398206061745No Hit
CGCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAA389460.1517541917195578No Hit
GGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAAG386640.1506553707349916No Hit
GGGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG374970.1461081221924783No Hit
CCGTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCG371690.1448300609054651No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAAAA370090.1442066163752148No Hit
GCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAA368540.14360265448653478No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA364440.1420050778777683No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA361610.14090236036488807No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAAA354790.13824492805469604No Hit
GGCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG354640.13818648012998508No Hit
CCGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAA352440.13732924390089085No Hit
CCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAAA352010.13716169318338609No Hit
GGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAAA346160.13488222411965833No Hit
GCGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG323900.12620855209255066No Hit
CGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA322920.12582669231777233No Hit
CGCTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCG321830.1254019707315393No Hit
CGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA318090.12394466914207915No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA317130.12357060242392895No Hit
GCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA313820.1222808515519736No Hit
CGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAAAA313220.12204705985312972No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGAT300130.11694650429001922No Hit
GCCTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCG294040.11457351854675393No Hit
CGGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA289160.11267201272949041No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGAT282620.1101236832120922No Hit
CGCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAAA282170.1099483394379593No Hit
CGGTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAA278210.10840531422558973No Hit
GCGTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAA268950.10479712900676598No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA261890.1020461800170364No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGAAAAAAAA261820.10201890431883795No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA260230.10139935631690168No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)