FastQCFastQC Report
Sat 23 Sept 2023
SRR19918541_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19918541_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25764301
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1796660.6973447484564009No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1356540.5265192329494987No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1330930.5165791224066199No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1319150.5120069044372677No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA1235900.47969475282873No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1202750.4668281122783032No Hit
CGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA1112900.43195427657827784No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA1086430.4216803708355993No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA983350.3816715229340008No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA936510.3634913285635034No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA930640.36121298225789245No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA837180.3249379829866139No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA829600.32199592762093565No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA799450.31029368893027603No Hit
CCGTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAA731720.28400537627626693No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA724950.2813777094127258No Hit
CGCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA713370.27688311823402467No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA706370.2741661805612347No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA698530.27112321036771No Hit
GGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA692510.2687866439691106No Hit
CCGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG658590.255621140274677No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAAA604910.23478610966391053No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA585320.2271825655196312No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA574280.22289756667568822No Hit
CCGCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA573810.22271514371765802No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA572520.22221445091795813No Hit
GGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA572460.2221911628807628No Hit
CCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA569710.22112379450930963No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA565180.21936554770106126No Hit
CGCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG551890.21420724746229286No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA533160.20693749851781346No Hit
CGGCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA521560.20243514466004725No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAA521360.20235751786939613No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA514940.19986569788949443No Hit
CCCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG507240.19687706644942551No Hit
GCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA503280.19534005599453289No Hit
GGCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA502240.19493639668314697No Hit
CCGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA493340.19148200449917116No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA482660.1873367338784002No Hit
CGGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG479530.18612187460470983No Hit
GCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA472060.1832225139738897No Hit
GGGCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA456250.17708611617291695No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAA444460.17251001686403214No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA442490.1717453929761184No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA440080.17080999014877213No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAA431770.1675845969972172No Hit
GGGGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA428710.16639690710025473No Hit
GCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA428100.16616014538876875No Hit
GGGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAA423990.16456491484088778No Hit
CCGGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA420430.16318315796729746No Hit
GCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA410060.15915820887203577No Hit
GGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAAG406430.15774928262171753No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA406250.1576794185101315No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAAA400940.1556184272183437No Hit
CGCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAA399540.15507503968378572No Hit
GGGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG386090.14985463801249643No Hit
CCGTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCG385010.14943545334298028No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAAAA384170.1491094208222455No Hit
GCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAA383660.14891147250608508No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA374370.14530570808033955No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA371060.14402098469506314No Hit
CCGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAA369440.1433922076907889No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAAA366760.1423520086960636No Hit
GGCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG362040.14052001643669665No Hit
CCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAAA361170.14018233989736417No Hit
GGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAAA352050.13664255824367214No Hit
CGCTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCG338140.13124361495388523No Hit
CGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA337050.1308205489448365No Hit
GCGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG335710.13030044944747385No Hit
CGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA328980.12768830794206293No Hit
CGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAAAA325210.12622504293828893No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA321880.1249325568739474No Hit
GCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA318800.12373710429791983No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGAT306040.11878451505437698No Hit
GCCTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCG301650.11708060699958443No Hit
CGGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA300850.11677009983697985No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGAT290550.11277232011844605No Hit
CGCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAAA288130.11183303595156725No Hit
CGGTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAA284650.11048232979423739No Hit
GCGTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAA273740.1062477883642176No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGAAAAAAAA269050.10442744012344833No Hit
GCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA267850.10396167937954147No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA266750.1035347320309602No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA262590.10192009478541646No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG262430.10185799335289554No Hit
CCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA259340.1006586594373354No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)