FastQCFastQC Report
Sat 23 Sept 2023
SRR19918545_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19918545_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12681124
Total Bases634 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA2397491.8905973949943238No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA2208171.7413046351411752No Hit
CGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA1931361.5230195682969427No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1595211.2579405421790688No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1288681.016219067016457No Hit
CGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA1260330.9938630045727808No Hit
GCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA1050880.8286962575241754No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1007500.7944879334040105No Hit
GGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA999350.7880610583099732No Hit
CGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA991960.7822334991756252No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG949490.7487427770598253No Hit
CCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA902310.7115378731412136No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA865150.6822344770069277No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG834250.6578675518037675No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA745630.5879841566094615No Hit
GCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA730270.5758716656346866No Hit
CGCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA727660.5738134884573323No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA683560.539037391322725No Hit
CCCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA682440.5381541888558143No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA636690.5020769452297761No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA630040.4968329305824941No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA588770.46428849682409856No Hit
GGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA563160.4440931261298289No Hit
GCCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA555050.4376977939810383No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA422480.3331565876968004No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA406770.320768095951116No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA392580.3095782361248104No Hit
GCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA369990.2917643577966748No Hit
CGGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA357750.28211221655115115No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA341130.26900612280110187No Hit
CGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA334250.26358073621865064No Hit
CGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAGATCGGAAG321910.25384973761000995No Hit
GGCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA309570.2441187390013693No Hit
CGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAAAA306230.2414849030732607No Hit
GCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA303870.23962386930369894No Hit
CGCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG300140.23668248965943395No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA269910.2128439087891578No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA262620.20709520701792677No Hit
CCGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA261860.20649589105823743No Hit
CGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAGATCGGA258630.20394879822955755No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA253840.20017153053625217No Hit
CGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAA253480.19988764402903086No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA251980.19870478358227553No Hit
GGGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAA251690.19847609722923615No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA245650.19371311249696793No Hit
CCCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG240440.189604643878571No Hit
CGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGAAAAAA237370.1871837228308784No Hit
CGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGAG234180.18466817294744534No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAGATCGGAAGA231670.18268885313320807No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAGATCGGAAG226680.1787538707136686No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA219970.17346254164851632No Hit
CGCCCCCTTGTAAATTTAACTGTTAGTCCAAAAAAAAAAAAAAAGATCGG207850.1639050292387331No Hit
GGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA207590.16370000009462884No Hit
GGGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA204550.16130273625587133No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAGATCGGAA202600.15976501767508935No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA200390.15802226995020313No Hit
CCGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG196830.15521494782323714No Hit
GGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGAG196420.15489163263445732No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAGATCGGAAGA196310.15480488953502858No Hit
GGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA193420.15252591174094662No Hit
GCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA192310.15165059501034767No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA186930.14740806887465183No Hit
CCGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA186100.1467535527607805No Hit
CCGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAA183990.14508966239901133No Hit
CCGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG179560.1415962812129272No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA179470.14152530958612186No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA177010.13958541845344308No Hit
CCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA175190.13815021444471326No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAGATCGGAA173850.13709352577894515No Hit
CGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAAA171590.13531134937250042No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAGATCGGA170510.13445968985083656No Hit
CGCCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA167190.13184162539535138No Hit
GCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAAAA165570.1305641361128556No Hit
GCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA162450.12810378638360448No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAGATCGGAAG160760.1267710969469268No Hit
GGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAAAA160750.1267632112106151No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA160200.12632949571347143No Hit
GGGGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA152670.1203915362707596No Hit
CGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGAAAAA149080.11756055693485845No Hit
CCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAAAA144730.11413026163926794No Hit
CGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAA143570.11321551622711047No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA142560.11241905685962852No Hit
CGGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA138430.10916224776289546No Hit
GCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAA138280.10904396171821994No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA137440.10838155986803694No Hit
CGCCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGA136570.10769550080891882No Hit
CCCCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA134040.10570040952205814No Hit
GGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAA132670.10462006364735492No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAGATCGGA131450.10365800381732723No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA130520.10292463034033891No Hit
CCCCCCCTTGTAAATTTAACTGTTAGTCCAAAAAAAAAAAAAAAGATCGG130170.10264862956942933No Hit
GCCCCCCTTGTAAATTTAACTGTTAGTCCAAAAAAAAAAAAAAAGATCGG129760.10232531438064954No Hit
GCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGAAAAAA129290.10195468477399952No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGAT127150.10026713720329523No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)