FastQCFastQC Report
Sat 23 Sept 2023
SRR19918546_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19918546_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12445585
Total Bases622.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA2354041.8914659294842306No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA2158151.7340687480741162No Hit
CGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA1893951.5217846328637825No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1566041.2583096736714265No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1271131.0213501414356978No Hit
CGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA1251361.0054649901953183No Hit
GCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA1024520.8231995522910334No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA996640.8007980340016159No Hit
CGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA988100.7939361629043552No Hit
GGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA973410.7821327804197231No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG926840.7447138884994157No Hit
CCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA892770.7173387189111641No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA858010.689409135850183No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG814370.6543444924445094No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA722280.5803503812797871No Hit
GCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA717380.5764132421256212No Hit
CGCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA704350.5659436659666861No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA678400.5450928984053381No Hit
CCCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA676610.5436546373673877No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA625930.5029333695443002No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA616940.4957099244430856No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA575330.46227638154413797No Hit
GCCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA550400.44224518172508565No Hit
GGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA546680.43925616995906575No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA409240.3288234341736447No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA398110.31988050380918215No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA385280.30957162720755993No Hit
GCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA361880.2907697790019513No Hit
CGGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA344650.27692551213944544No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA332820.2674201333243877No Hit
CGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA324630.26083948645242466No Hit
CGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAGATCGGAAG318510.25592207999865013No Hit
GGCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA300670.24158767948633994No Hit
CGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAAAA297300.23887989194561768No Hit
GCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA294890.23694346227999727No Hit
CGCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG292700.2351838021274211No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA268210.21550614133445714No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA258200.20746312849094678No Hit
CCGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA256130.20579988807275834No Hit
CGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAGATCGGA251310.2019270287415176No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA247170.19860054790514065No Hit
CGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAA247060.19851216314861858No Hit
GGGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAA246470.19803809945454554No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA243370.19554725631619566No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA237490.19082268933119656No Hit
CCCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG237130.19053343012803337No Hit
CGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGAAAAAA227780.18302072582365553No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAGATCGGAAGA225420.18112447104736337No Hit
CGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGAG225220.1809637714900505No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAGATCGGAAG223910.17991118938965103No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA218170.17529911209477095No Hit
GGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA200730.16128611069708654No Hit
CGCCCCCTTGTAAATTTAACTGTTAGTCCAAAAAAAAAAAAAAAGATCGG199100.15997640930498647No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA195420.1570195374504292No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAGATCGGAA195340.15695525762750406No Hit
CCGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG194640.1563928091769089No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAGATCGGAAGA192370.15456886920140758No Hit
GGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGAG190200.15282527900456266No Hit
GCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA190000.15266457944724976No Hit
GGGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA188570.15151557761246257No Hit
GGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA186800.15009338653024346No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA182760.146847255472523No Hit
CCGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAA182090.14630891195552478No Hit
CCGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA179050.1438662786843688No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA177680.14276548671677547No Hit
CCGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG175140.14072460233890172No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA172530.13862747311596843No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAGATCGGAA171010.13740615648039045No Hit
CCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA169500.13619287482267808No Hit
CGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAAA169500.13619287482267808No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAGATCGGA166650.13390290613096933No Hit
CGCCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA164140.1318861266866925No Hit
GCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAAAA160160.1286882054961659No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAGATCGGAAG159310.1280052323775861No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA159230.12794095255466095No Hit
GCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA158920.12769186824082596No Hit
GGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAAAA153600.12341726001630297No Hit
GGGGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA149020.11973724015383769No Hit
CGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGAAAAA146790.11794544008979892No Hit
CCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAAAA143690.11545459695144905No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA143410.11522961757121099No Hit
CGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAA139150.11180671700044634No Hit
GCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAA135650.10899447474747069No Hit
CGCCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGA135180.10861683078778539No Hit
CCCCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA133750.1074678289529982No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA133060.1069134154802687No Hit
CGGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA131890.10597332306998827No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA129400.10397261358144273No Hit
GCCCCCCTTGTAAATTTAACTGTTAGTCCAAAAAAAAAAAAAAAGATCGG128560.10329767544072858No Hit
GCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGAAAAAA128120.10294413641464022No Hit
GGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAA127970.10282361174665554No Hit
CCCCCCCTTGTAAATTTAACTGTTAGTCCAAAAAAAAAAAAAAAGATCGG127950.10280754179092425No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAGATCGGA126600.10172281977906222No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)