FastQCFastQC Report
Sat 23 Sept 2023
SRR19918548_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19918548_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12558744
Total Bases627.9 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA2320601.8477962445926124No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA2128441.694787313126217No Hit
CGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA1871991.4905869567848504No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1550861.2348846349603113No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1249350.994804894502189No Hit
CGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA1217930.9697864690927692No Hit
GCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA1016990.809786392652004No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA980850.781009629625383No Hit
GGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA969080.7716376733214723No Hit
CGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA964270.7678076724869939No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG905910.7213380573726162No Hit
CCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA867540.6907856390734615No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA821590.6541975853636319No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG786690.6264081822194958No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA710900.566059790692445No Hit
GCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA710570.5657970255624288No Hit
CGCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA698740.5563772937803335No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA663110.5280066223182828No Hit
CCCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA653130.5200599677802175No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA611290.4867445343260441No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA603000.4801435557568495No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA565090.44995741612377804No Hit
GGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA538310.42863362769397956No Hit
GCCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA536840.4274631284784529No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA409330.325932274756138No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA398220.31708584871225975No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA381530.3037963031971987No Hit
GCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA355430.2830139701868276No Hit
CGGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA336030.2675665655737548No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA328850.26184943335097843No Hit
CGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA327310.2606231960775695No Hit
CGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAGATCGGAAG312390.24874302716895894No Hit
GGCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA299250.23828019744649623No Hit
CGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAAAA298750.23788206846162324No Hit
GCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA290530.23133682795031096No Hit
CGCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG288860.23000707714083513No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA265510.21141445354726554No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA254150.20236896301095078No Hit
CCGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA252180.20080033481055115No Hit
CGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAGATCGGA248150.19759141519247467No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA246720.19645276629573785No Hit
CGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAA243930.19423120656014647No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA241450.19225648679517632No Hit
GGGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAA237740.1893023697274186No Hit
CCCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG237060.1887609143079913No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA236980.18869721367041165No Hit
CGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGAAAAAA226440.18030465466928858No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAGATCGGAAGA221460.1763392899799534No Hit
CGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGAG221270.17618800096570167No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAGATCGGAAG215640.17170506859603157No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA213040.1696347978746919No Hit
GGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA199200.15861458757340702No Hit
CGCCCCCTTGTAAATTTAACTGTTAGTCCAAAAAAAAAAAAAAAGATCGG197360.15714947290907433No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA195310.15551714407109501No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAGATCGGAA193100.1537574139579563No Hit
CCGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG188990.15048479370230017No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAGATCGGAAGA186920.1488365397049259No Hit
GGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA186730.14868525069067418No Hit
GGGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA184700.14706884701208975No Hit
GCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA183600.14619296324536912No Hit
GGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGAG182410.14524541626137136No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA177010.14094562322474286No Hit
CCGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAA176850.1408182219495835No Hit
CCGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA175380.13964772273405684No Hit
CCGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG173700.13831000934488355No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA173220.13792780551940545No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA172420.13729079914360862No Hit
CCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA170060.13541163033500803No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAGATCGGAA165800.13201957138388998No Hit
CGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAAA164610.1310720243998922No Hit
CGCCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA163070.12984578712648334No Hit
GCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAAAA160240.1275923770721021No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAGATCGGA158800.12644576559566784No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA158670.12634225205960087No Hit
GCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA158180.1259520856544253No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAGATCGGAAG156970.12498861351103263No Hit
GGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAAAA152670.12156470424112475No Hit
GGGGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA146020.11626958874231372No Hit
CGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGAAAAA142900.11378526387670614No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA140030.11150000350353506No Hit
CCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAAAA138500.11028172880982365No Hit
CGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAA136440.10864143739214686No Hit
GCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAA133660.10642784023625293No Hit
CGGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA132180.1052493784410288No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA132000.10510605200647453No Hit
CCCCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA131320.10456459658704724No Hit
CGCCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGA131060.10435756951491329No Hit
GGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAA128460.10228729879357362No Hit
GCCCCCCTTGTAAATTTAACTGTTAGTCCAAAAAAAAAAAAAAAGATCGG127010.10113272473744189No Hit
CCCCCCCTTGTAAATTTAACTGTTAGTCCAAAAAAAAAAAAAAAGATCGG126800.10096551056379523No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA126290.10055941899922477No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)