FastQCFastQC Report
Sat 23 Sept 2023
SRR19918549_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19918549_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12574433
Total Bases628.7 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA2346681.866231264662192No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA2158621.716673825372484No Hit
CGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA1898181.5095551425658715No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1568501.247372346729272No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA1271471.0111549363696954No Hit
CGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA1222450.9721710712522783No Hit
GCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA1035130.8232021276824172No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA997560.7933240409328993No Hit
GGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA976400.7764962444032267No Hit
CGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA970790.7720348106351993No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG913430.7264184397022116No Hit
CCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAA878360.6985285141683923No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA829150.6593935487985819No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG798760.6352254610605504No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA725460.5769325742162689No Hit
GCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA715430.5689560714188863No Hit
CGCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA708660.5635721308467746No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA679610.5404696975203573No Hit
CCCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA668210.531403682376772No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA622590.4951237165127048No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA606420.48226428976956653No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA574030.4565056730589761No Hit
GCCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA552470.4393597707347918No Hit
GGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAA546790.4348426684527246No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA413300.32868281217928474No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA403160.3206188302884114No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA387300.3080059355360198No Hit
GCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA359650.28601687249039376No Hit
CGGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA337950.26875963313813034No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA331090.2633041187622535No Hit
CGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA328910.2615704421821644No Hit
CGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAGATCGGAAG318340.2531644965621909No Hit
GGCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA307210.24431320282990096No Hit
CGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAAAA298800.23762502850029105No Hit
CGCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG294820.2344598758448989No Hit
GCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA291080.23148558666621388No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA266110.21162783244381675No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA259470.2063472762549214No Hit
CCGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA256140.2036990455156109No Hit
CGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAGATCGGA250320.1990706062054647No Hit
CGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAA249810.1986650213174622No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA247460.19679614977470555No Hit
GGGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAA244990.1948318464935954No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA243840.19391729233437405No Hit
CCCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG242060.19250172154879666No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA236840.18835044093041808No Hit
CGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGAAAAAA233110.18538410439659586No Hit
CGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGAG224840.17880726709506503No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAGATCGGAAGA222140.17666005298211063No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAGATCGGAAG220140.17506952400955178No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA219200.1743219753924491No Hit
GGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA204090.16230552900476705No Hit
CGCCCCCTTGTAAATTTAACTGTTAGTCCAAAAAAAAAAAAAAAGATCGG195960.15584002873131536No Hit
CCGCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAG194480.1546630372916218No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA194150.1544006000111496No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAGATCGGAA191150.15201480655231134No Hit
GCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA187540.14914390175684264No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAGATCGGAAGA187350.14899280150444955No Hit
GGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA186820.14857131132672144No Hit
GGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGAG186560.1483645425602888No Hit
GGGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA185700.1476806151020885No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA180260.14335437629672845No Hit
CCGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA178820.14220919543648608No Hit
CCGGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAGATCGGAA178490.14194675815601387No Hit
CCGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG176590.14043575563208296No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA175680.1397120649495687No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA174350.13865436318281707No Hit
CCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA170450.1355528316863273No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAGATCGGAA167610.13329428054529377No Hit
CGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAAA167290.13303979590968434No Hit
CGCCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA163190.12977921151593874No Hit
GCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAAAA162840.12950086894574095No Hit
GCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA162320.12908733141287562No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAGATCGGA160440.12759223417867033No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA160020.12725822309443297No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAGATCGGAAG158630.12615280545850457No Hit
GGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAAAA156500.1244588921027294No Hit
GGGGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA147860.1175878069412752No Hit
CGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGAAAAA144940.11526563464133929No Hit
CCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAAAA141800.1127685041544219No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA140010.11134498072398175No Hit
CGCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAA136760.10876037114357365No Hit
CGCCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGA135870.10805258575078494No Hit
GCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAA135800.1079969172367454No Hit
CGGCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA135490.10775038524599877No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA134320.10681992579705184No Hit
CCCCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA131160.10430689002040887No Hit
GGCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAA129160.10271636104785002No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAAAA128810.10243801847765223No Hit
GCCCCCCTTGTAAATTTAACTGTTAGTCCAAAAAAAAAAAAAAAGATCGG128740.10238234996361269No Hit
GCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGAAAAAA127320.10125307439309589No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)