FastQCFastQC Report
Fri 30 Jun 2023
SRR2007064.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007064.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19223712
Total Bases980.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG10764035.59935042722238TruSeq Adapter, Index 6 (100% over 49bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA3720011.9351153408873376No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG3327251.7308051639558477Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC3065221.5944995430643156Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG2807751.4605659926657246No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC2752481.4318150417567637No Hit
CGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT2212661.15100559142792TruSeq Adapter, Index 6 (100% over 43bp)
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC2048351.0655330250473998Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC2024491.0531212702312645TruSeq Adapter, Index 6 (100% over 50bp)
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA1870700.9731211120932315TruSeq Adapter, Index 6 (100% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1716240.8927724260538235No Hit
ATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACACG843610.438838243103101No Hit
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT810420.42157310721259245Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC793000.41251138177683894TruSeq Adapter, Index 6 (100% over 42bp)
CCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC674300.3507647222347068TruSeq Adapter, Index 6 (100% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA632690.3291195789866182No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACA567170.29503667137751544No Hit
TCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA474760.24696583053262555Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACAC474200.24667452362998363No Hit
ACGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC415770.2162797694846864TruSeq Adapter, Index 6 (100% over 42bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC405630.21100503378327765No Hit
TGGATGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA394690.20531414536380904TruSeq Adapter, Index 6 (100% over 40bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG381990.19870772096460873No Hit
GTTTCTGTAGTGTAGTGGTTATCACGCTCGCCTGAGATCGGAAGAGCACA377100.1961639874754678No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC373900.19449937660322836Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC370160.19255386264629848TruSeq Adapter, Index 6 (100% over 44bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA327780.17050817240707725No Hit
ATATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACA320820.16688764375995646No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA312890.16276252994218807No Hit
GTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT302250.1572276987919919Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA298900.15548505928511622No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC271960.14147111650445035No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCTGAGATCGGAAGAGCACA258710.13457858711158388No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC240100.1248978345077163No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC239880.12478339251024984No Hit
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT232680.12103801804771107TruSeq Adapter, Index 6 (100% over 43bp)
GACGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAT227570.1183798425611037TruSeq Adapter, Index 6 (100% over 41bp)
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC225190.11714178822487561No Hit
TTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCAGATCGGAAGAGC214610.11163816852853392No Hit
TGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCC211240.10988512520370675Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC207160.10776274634160146TruSeq Adapter, Index 6 (100% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)