FastQCFastQC Report
Fri 30 Jun 2023
SRR2007066.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007066.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22014222
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG17753058.06435494290918No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC5593392.5408074834531966Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC5569292.5298600150393686Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG4132131.8770274961340898No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG4114591.8690599195374697TruSeq Adapter, Index 7 (100% over 49bp)
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC3774681.7146551897223532Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA3179501.444293602562925TruSeq Adapter, Index 7 (100% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC2640971.199665379953014No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG2531131.1497703620868363No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2308861.0488038141888458No Hit
CCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA2214291.005845221330102TruSeq Adapter, Index 7 (100% over 35bp)
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1599730.7266802342594709Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACACG1260280.5724844602729998No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1228890.5582254962269391No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC1065170.48385539130113253Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1060130.48156596222205805No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCAGATCGGAAGAGCACACG897390.40764102406162706No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTC874980.3974612411921711TruSeq Adapter, Index 7 (100% over 50bp)
TGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCC854620.3882126745155927Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA851430.38676361126911507Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACAC725070.329364353643749No Hit
TCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTC709310.3222053452536274Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATATTAAATGGATTTTTGGAAGTAGGAGTAGATCGGAAGAGCACACGTCT707900.3215648502136482Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG673370.30587953551118No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA636560.28915852670151143No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC612090.27804298512116393No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC603500.27414096214710654No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT595620.2705614579520457No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACA592100.26896249161110486No Hit
GTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT576620.26193067372537626Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT576030.2616626651625481No Hit
CGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT556010.2525685440984469TruSeq Adapter, Index 7 (100% over 43bp)
TATATTAAATGGATTTTTGGAAGTAGGAGTAGATCGGAAGAGCACACGTC544290.24724471298599604No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC541390.24592738276192544No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC524270.23815059192189486No Hit
TGTGGGGGGAACCTCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAAC520590.23647894529272942Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGAACCTCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAACTC520150.23627907450011182Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGTTGAACGCACGTTCGTGTGGAACCTGGCGCTAGATCGGAAGAGCACA505830.22977418870401145No Hit
ATATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACA485540.22055741965353123No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACAC484050.2198805844694398No Hit
ACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG455120.20673907985483206TruSeq Adapter, Index 7 (100% over 36bp)
TCCCGTGGATCGCCTCAGTAGATCGGAAGAGCACACGTCTGAACTCCAGT440250.19998435556795963Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGCGTCGGGGTCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC415940.18894149427583679Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTAGATCGGAAGAGCACAC415670.1888188462894578No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC414960.1884963275104612No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG395140.1794930568066407No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG385920.175304855197699No Hit
ACCTCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC380300.17275196007381047Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA378600.17197973201142425Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATATATTAAATGGATTTTTGGAAGTAGGAGTAGATCGGAAGAGCACACGT367730.16704201493016652No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC360470.16374414685197597No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC355000.16125938949829796No Hit
GTTTCTGTAGTGTAGTGGTTATCACGCTCGCCTGAGATCGGAAGAGCACA349000.15853387868987603No Hit
ATGGTTAGCACTCTGGACTCAGATCGGAAGAGCACACGTCTGAACTCCAG334050.15174281425889136Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC334040.15173827174087734No Hit
TCGCTGCGATCTATTGAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT331140.15042094151680674Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA312330.14187646513240396TruSeq Adapter, Index 7 (100% over 37bp)
TTGAACGCACGTTCGTGTGGAACCTGGCGCTAGATCGGAAGAGCACACGT291990.13263698349185357No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT282410.12828525123440654No Hit
TCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA281370.12781282936094676TruSeq Adapter, Index 7 (100% over 35bp)
TGGGGGGAACCTCCGCGTCGGTGTAGATCGGAAGAGCACACGTCTGAACT275150.12498738315621602Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAG273360.12417427243170347Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCAGATCGGAAGAGC270680.12295687760394167No Hit
CGCGTCGGGGTCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA261640.11885044131925261Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT259270.11777386454992596TruSeq Adapter, Index 7 (100% over 43bp)
TTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCAAGATCGGAAGAG255690.11614764310090087No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA250140.11362654560311057No Hit
TGTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACT248500.11288157264880856Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGGTTAGCACTCTGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGT241870.10986988320550234Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACTAGATCGGAAGAGCA241750.10981537298933389No Hit
TCGCTGCGATCTATTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC240410.109206675575453Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG238260.10823003420243514TruSeq Adapter, Index 7 (100% over 36bp)
TCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAACTCC234330.10644482462291877Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC232070.1054182155517465No Hit
TGGGGGGAACCTCCGCGTCGGCAGATCGGAAGAGCACACGTCTGAACTCC228830.10394643971519865Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA226230.10276538503154915No Hit
GTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAA221670.10069399681714847Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)