FastQCFastQC Report
Fri 30 Jun 2023
SRR2007067.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007067.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21063979
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG12875146.112396902788405No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA7067503.355254009700636No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC5861272.782603419800219No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG3290751.5622641856982482TruSeq Adapter, Index 3 (100% over 49bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA2905901.379558914296297No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC2256441.0712316034876412No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1936020.9191140952048994No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC1785760.8477790449753106Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC1531670.7271513136240784Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA1399250.6642856983478763No Hit
ACGTTGAACGCACGTTCGTGTGGAACCTGGCGCTAGATCGGAAGAGCACA1090380.5176514845556958No Hit
ATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACACG969530.46027865865228973No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTC896820.4257600142878988TruSeq Adapter, Index 3 (100% over 50bp)
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT884690.42000136821253004Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG787750.37397967402075366No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACA766910.36408600673215635No Hit
TCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTC710010.33707306677432597Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC698200.331466338814713No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA673290.3196404629913465No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC663240.3148692846683905No Hit
GTTTCTGTAGTGTAGTGGTTATCACGCTCGCCTGAGATCGGAAGAGCACA662490.31451322658458786No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC658040.3124006152873586No Hit
TCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACG577600.27421219893924126No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC561150.266402658301169Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT561100.2663789210955822No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC518960.24637320422698866No Hit
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG515730.24483978074607843TruSeq Adapter, Index 3 (100% over 37bp)
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACAC506490.24045314515362934No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCTGAGATCGGAAGAGCACA499190.23698751313794986No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC487480.23142825958951063No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC448700.21301768293635312No Hit
TATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACAC447530.21246223232562092No Hit
TTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACAC440160.20896336822211983No Hit
TCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCAC427220.2028201794162442No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC424670.20160958193131506No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG418910.19887505584771045No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA397280.188606340710841No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC386710.18358829544978184No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT386060.18327971177715283Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT371430.1763342054224418TruSeq Adapter, Index 3 (100% over 35bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTAGATCGGAAGAGCACACG368190.17479603450041417No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG365640.17358543701548507No Hit
ATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT343510.16307934982274716Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA340690.16174057142764908No Hit
TGGGGCGAAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACA340120.16146996728395901No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT335630.15933836622226027No Hit
ATATTAAATGGATTTTTGGAACTAGGAGTTGGAGATCGGAAGAGCACACG335350.15920543787097394No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC304290.14445988576042543No Hit
ATATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACA283030.13436682594489863No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG282860.13428611944590335No Hit
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT271110.12870787613299461Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC268790.127606469793765No Hit
TCCGGAGCGGGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT261050.12393195036892127Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGTTGAACGCACGTTCGTGTGGAACCTGGCGCAGATCGGAAGAGCACAC251640.11946460827747692No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCA245700.11664462825375967No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC239700.11379616358333816No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT237110.11256657633393957No Hit
TCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA232530.1103922483021845Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCA230050.10921488290507694No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC228170.10832236397501156No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCA221890.10534097095330375No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA220030.10445794690547307No Hit
TCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT216680.10286755413115442TruSeq Adapter, Index 3 (100% over 35bp)
TCGCGTCGGGGTCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC213970.10158099758834739Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAGATCGGAAGAGC213850.10152402829493895No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGT213570.10139109994365261No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC213430.10132463576800946No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)